دورية أكاديمية
A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells
| العنوان: | A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells |
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| المؤلفون: | Cuevas-Ocaña, Sara, Yang, Jin Ye, Aushev, Magomet, Schlossmacher, George, Bear, Christine E., Hannan, Nicholas R.F., Perkins, Neil D., Rossant, Janet, Wong, Amy P., Gray, Michael A. |
| المساهمون: | Cuevas Ocana, Sara |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2023 |
| المجموعة: | University of Nottingham: Repository@Nottingham |
| مصطلحات موضوعية: | stem cells, gene editing, TALEN, CRISPR-Cas9, ΔF508, W1282X, cystic fibrosis, Bio/Medical/Health - Biochemistry & Molecular Biology, Bio/Medical/Health - Cell & Developmental Biology, Bio/Medical/Health - Genetics & Systems Biology, Bio/Medical/Health - Human Biology, Biodiscovery Institute (BDI) |
| الوصف: | Introducing or correcting disease-causing mutations through genome editing in human pluripotent stem cells (hPSCs) followed by tissue-specific differentiation provide sustainable models of multiorgan diseases, such as cystic fibrosis (CF). However, low editing efficiency resulting in extended cell culture periods and the use of specialised equipment for fluorescence activated cell sorting (FACS) make hPSC genome editing still challenging. We aimed to investigate whether a combination of cell cycle synchronisation, single-stranded oligodeoxyribonucleotides, transient selection, manual clonal isolation, and rapid screening can improve the generation of correctly modified hPSCs. Here, we introduced the most common CF mutation, ΔF508, into the CFTR gene, using TALENs into hPSCs, and corrected the W1282X mutation using CRISPR-Cas9, in human-induced PSCs. This relatively simple method achieved up to 10% efficiency without the need for FACS, generating heterozygous and homozygous gene edited hPSCs within 3–6 weeks in order to understand genetic determinants of disease and precision medicine. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1661-6596 |
| العلاقة: | https://nottingham-repository.worktribe.com/output/22176332Test; International Journal of Molecular Sciences; Volume 24; Issue 12; https://nottingham-repository.worktribe.com/file/22176332/1/Cell-Based%20Optimised%20Approach%20for%20Rapid%20and%20Efficient%20Gene%20EditingTest |
| DOI: | 10.3390/ijms241210266 |
| الإتاحة: | https://doi.org/10.3390/ijms241210266Test https://nottingham-repository.worktribe.com/file/22176332/1/Cell-Based%20Optimised%20Approach%20for%20Rapid%20and%20Efficient%20Gene%20EditingTest https://nottingham-repository.worktribe.com/output/22176332Test |
| حقوق: | openAccess ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.4909B8C2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16616596 |
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| DOI: | 10.3390/ijms241210266 |