التفاصيل البيبلوغرافية
| العنوان: |
Improved Neural Inductivity of Size-Controlled 3D Human Embryonic Stem Cells Using Magnetic Nanoparticles |
| المؤلفون: |
Son, Boram, Park, Sora, Cho, Sungwoo, Kim, Jeong Ah, Baek, Seung-Ho, Yoo, Ki Hyun, Han, Dongoh, Joo, Jinmyoung, Park, Hee Ho, Park, Tai Hyun |
| المساهمون: |
National Research Foundation of Korea, Korea Evaluation Institute of Industrial Technology, Institute of Information & Communications Technology Planning & Evaluation, Hanyang University |
| المصدر: |
Biomaterials Research ; volume 28 ; ISSN 2055-7124 |
| بيانات النشر: |
American Association for the Advancement of Science (AAAS) |
| سنة النشر: |
2024 |
| مصطلحات موضوعية: |
Biomedical Engineering, Biomaterials, Medicine (miscellaneous), Ceramics and Composites |
| الوصف: |
Background: To improve the efficiency of neural development from human embryonic stem cells, human embryoid body (hEB) generation is vital through 3-dimensional formation. However, conventional approaches still have limitations: long-term cultivation and laborious steps for lineage determination. Methods: In this study, we controlled the size of hEBs for ectodermal lineage specification using cell-penetrating magnetic nanoparticles (MNPs), which resulted in reduced time required for initial neural induction. The magnetized cells were applied to concentrated magnetic force for magnet-derived multicellular organization. The uniformly sized hEBs were differentiated in neural induction medium (NIM) and suspended condition. This neurally induced MNP-hEBs were compared with other groups. Results: As a result, the uniformly sized MNP-hEBs in NIM showed significantly improved neural inductivity through morphological analysis and expression of neural markers. Signaling pathways of the accelerated neural induction were detected via expression of representative proteins; Wnt signaling, dopaminergic neuronal pathway, intercellular communications, and mechanotransduction. Consequently, we could shorten the time necessary for early neurogenesis, thereby enhancing the neural induction efficiency. Conclusion: Overall, this study suggests not only the importance of size regulation of hEBs at initial differentiation stage but also the efficacy of MNP-based neural induction method and stimulations for enhanced neural tissue regeneration. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.34133/bmr.0011 |
| الإتاحة: |
https://doi.org/10.34133/bmr.0011Test |
| رقم الانضمام: |
edsbas.48FDBF0A |
| قاعدة البيانات: |
BASE |
