دورية أكاديمية
Invasive Streptococcus suis isolated in Spain contain a highly promiscuous and dynamic resistome
| العنوان: | Invasive Streptococcus suis isolated in Spain contain a highly promiscuous and dynamic resistome |
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| المؤلفون: | Cristina Uruén, Jorge Gimeno, Marina Sanz, Lorenzo Fraile, Clara M. Marín, Jesús Arenas |
| المصدر: | Frontiers in Cellular and Infection Microbiology, Vol 13 (2024) |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Streptococcus suis, antimicrobial resistance (AMR), antimicrobial resistant genes, mobile genetic elements, ICEs, IMEs, Microbiology, QR1-502 |
| الوصف: | IntroductionStreptococcus suis is a major pathogen for swine and human. Here we aimed to know the rates of antimicrobial resistance (AMR) in invasive S. suis isolates recovered along Spain between 2016 – 2021 and elucidate their genetic origin.MethodsAntibiotic susceptibility testing was performed for 116 isolates of different genetic backgrounds and geographic origins against 18 antibiotics of 9 families. The association between AMR and genotypes and the origin of the isolates were statistically analyzed using Pearson´s chi-square test and the likelihood ratio. The antimicrobial resistant genes were identified by whole genome sequencing analysis and PCR screenings.ResultsHigh AMR rates (>80%) were detected for tetracyclines, spectinomycin, lincosamides, and marbofloxacin, medium (20-40%) for sulphonamides/trimethoprim, tiamulin, penicillin G, and enrofloxacin, and low (< 20%) for florfenicol, and four additional β-lactams. The occurrence of multidrug resistance was observed in 90% of isolates. For certain antibiotics (penicillin G, enrofloxacin, marbofloxacin, tilmicosin, and erythromycin), AMR was significantly associated with particular sequence types (STs), geographic regions, age of pigs, and time course. Whole genome sequencing comparisons and PCR screenings identified 23 AMR genes, of which 19 were previously reported in S. suis (aph(3’)-IIIa, sat4, aadE, spw, aac(6’)-Ie-aph(2’’)-Ia, fexA, optrA, erm(B), mef(A/E), mrs(D), mph(C), lnu(B), lsa(E), vga(F), tet(M), tet(O), tet(O/W/32/O), tet(W)), and 4 were novel (aph(2’’)-IIIa, apmA, erm(47), tet(T)). These AMR genes explained the AMR to spectinomycin, macrolides, lincosamides, tiamulin, and tetracyclines. Several genes were located on mobile genetic elements which showed a variable organization and composition. As AMR gene homologs were identified in many human and animal pathogens, the resistome of S. suis has a different phylogenetic origin. Moreover, AMR to penicillin G, fluoroquinolones, and trimethoprim related to mutations in genes coding for ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2235-2988 |
| العلاقة: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1329632/fullTest; https://doaj.org/toc/2235-2988Test; https://doaj.org/article/7897ea8057454a2684e1412e8c6916cbTest |
| DOI: | 10.3389/fcimb.2023.1329632 |
| الإتاحة: | https://doi.org/10.3389/fcimb.2023.1329632Test https://doaj.org/article/7897ea8057454a2684e1412e8c6916cbTest |
| رقم الانضمام: | edsbas.48DC5641 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22352988 |
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| DOI: | 10.3389/fcimb.2023.1329632 |