دورية أكاديمية
Unlocking Predictive Power: Quantitative Assessment of CAR-T Expansion with Digital Droplet Polymerase Chain Reaction (ddPCR)
| العنوان: | Unlocking Predictive Power: Quantitative Assessment of CAR-T Expansion with Digital Droplet Polymerase Chain Reaction (ddPCR) |
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| المؤلفون: | Galli E., Viscovo M., Pansini I., Hohaus S., Bellesi S., Chiusolo P. |
| المساهمون: | Galli, Eugenio, Viscovo, Marcello, Fosso, F., Pansini, Ilaria, Di Cesare, G., Iacovelli, C., Maiolo, E., Sora, F., Hohaus, Stefan, Sica, S., Bellesi, Silvia, Chiusolo, Patrizia |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute (MDPI) |
| سنة النشر: | 2024 |
| المجموعة: | Università Cattolica del Sacro Cuore: PubliCatt |
| مصطلحات موضوعية: | B-cell lymphoma, CAR-T cells, digital droplet PCR, Settore MED/15 - MALATTIE DEL SANGUE |
| الوصف: | Flow cytometry (FCM) and quantitative PCR (qPCR) are conventional methods for assessing CAR-T expansion, while digital droplet PCR (ddPCR) is emerging as a promising alternative. We monitored CAR-T transcript expansion in 40 B-NHL patients post-infusion of CAR-T products (axi-cel; tisa-cel; and brexu-cel) with both His-Tag FCM and ddPCR techniques. Sensitivity and predictive capacity for efficacy and safety outcomes of ddPCR were analyzed and compared with FCM. A significant correlation between CAR-T counts determined by FCM and CAR transcripts assessed by ddPCR (p < 0.001) was observed. FCM revealed median CD3+CAR+ cell counts at 7, 14, and 30 days post-infusion with no significant differences. In contrast, ddPCR-measured median copies of CAR-T transcripts demonstrated significant lower copy numbers in tisa-cel recipients compared to the other products at day 7 and day 14. Patients with a peak of CAR transcripts at day 7 exceeding 5000 copies/microg gDNA, termed “good CAR-T expanders”, were more likely to achieve a favorable response at 3 months (HR 10.79, 95% CI 1.16–100.42, p = 0.036). Good CAR-T expanders showed superior progression-free survival at 3, 6, and 12 months compared to poor CAR-T expanders (p = 0.088). Those reaching a peak higher than 5000 copies/microg gDNA were more likely to experience severe CRS and ICANS. DdPCR proves to be a practical method for monitoring CAR-T expansion, providing quantitative information that better predicts both treatment outcomes and toxicity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/38473919; info:eu-repo/semantics/altIdentifier/wos/WOS:001183352800001; volume:25; issue:5; firstpage:N/A; lastpage:N/A; issueyear:2024; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; https://hdl.handle.net/10807/274574Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85187782506 |
| DOI: | 10.3390/ijms25052673 |
| الإتاحة: | https://doi.org/10.3390/ijms25052673Test https://hdl.handle.net/10807/274574Test |
| رقم الانضمام: | edsbas.483FB018 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms25052673 |
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