التفاصيل البيبلوغرافية
| العنوان: |
Two-step nature of human T-cell leukemia virus type 1 replication in experimentally infected squirrel monkeys (Saimiri sciureus). |
| المؤلفون: |
Mortreux, F., Kazanji, M., Gabet, As, de Thoisy, B., Wattel, E. |
| المساهمون: |
Virologie et pathogenèse virale (VPV), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire et approches thérapeutiques des hémopathies malignes, IRCL-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Rétrovirologie Cayenne, Guyane française, Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre de Primatologie Cayenne, Guyane française (CP-IPG), This work was supported by grants from the Association pour la Recherche sur le Cancer (ARC), the Fondation contre la Leuce´mie, the comite´ de´partemental du Rhoˆne de la Ligue Nationale Contre le Cancer, and the Association Virus Cancer Pre´vention (VCP). F.M. was supported by funds from the Ministe`re de l'Enseignement Supe rieur et de la Recherche and from the Fondation pour la Recherche Medicale. |
| المصدر: |
https://hal.science/hal-00117192Test ; 2006. |
| بيانات النشر: |
HAL CCSD |
| سنة النشر: |
2006 |
| المجموعة: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: |
Animals, Cell Line, Clone Cells, DNA, Viral/analysis, HTLV-I Infections/physiopathology/*virology, Human T-lymphotropic virus 1/genetics/isolation & purification/*physiology, Humans, Leukocytes, Mononuclear/virology, Lymphocyte Activation, Lymphoid Tissue/virology, Male, Proviruses/genetics, Research Support, Non-U.S. Gov't, Saimiri, Viral Load, Virus Replication, OCIS 000.1430, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: |
After experimental infection of squirrel monkeys (Saimiri sciureus) with human T-cell leukemia virus type 1 (HTLV-1)-infected cells, the virus is transcribed only transiently in circulating blood, spleen, and lymph nodes. Stable disappearance of viral expression occurs at 2 to 3 weeks after inoculation. This coincides with the development of the anti-HTLV-1 immune response and persistent detection of the provirus in peripheral blood mononuclear cells (PBMCs). In this study, the HTLV-1 replication pattern was analyzed over time in PBMCs and various organs from two HTLV-1-infected squirrel monkeys. Real-time quantitative PCR confirmed that PBMCs and lymphoid organs constitute the major reservoirs for HTLV-1. The PCR amplification of HTLV-1 flanking sequences from PBMCs evidenced a pattern of clonal expansion of infected cells identical to that observed in humans. Dissemination of the virus in body compartments appeared to result from cellular transport of the integrated provirus. The circulating proviral burden increased as a function of time in one animal studied over a period of 4 years. The high proviral loads observed in the last samples resulted from the accumulation of infected cells via the extensive proliferation of a restricted number of persistent clones on a background of polyclonally expanded HTLV-1-positive cells. Therefore, HTLV-1 primary infection in squirrel monkeys is a two-step process involving a transient phase of reverse transcription followed by persistent multiplication of infected cells. This suggests that the choice of the target for blocking HTLV-1 replication might depend on the stage of infection. |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
info:eu-repo/semantics/altIdentifier/pmid/11134325; hal-00117192; https://hal.science/hal-00117192Test; PUBMED: 11134325 |
| الإتاحة: |
https://hal.science/hal-00117192Test |
| رقم الانضمام: |
edsbas.47FE1159 |
| قاعدة البيانات: |
BASE |
