كتاب
Miniature Multiphoton Microscopes for Recording Neural Activity in Freely Moving Animals
| العنوان: | Miniature Multiphoton Microscopes for Recording Neural Activity in Freely Moving Animals |
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| المؤلفون: | Ozbay, Baris N., Futia, Gregory L., Ma, Ming, McCullough, Connor, Young, Michael D., Restrepo, Diego, Gibson, Emily A. |
| المصدر: | Neuromethods ; All-Optical Methods to Study Neuronal Function ; page 187-230 ; ISSN 0893-2336 1940-6045 ; ISBN 9781071627631 9781071627648 |
| بيانات النشر: | Springer US |
| سنة النشر: | 2023 |
| الوصف: | Miniaturized head-mounted microscopes for in vivo recording of neural activity have gained much recognition within the past decade of neuroscience research. In combination with fluorescent reporters, these miniature microscopes allow researchers to record the neural activity that underlies behavior, cognition, and perception in freely moving animals. Single-photon miniature microscopes are convenient for widefield recording but lack the increased penetration depth and optical sectioning capabilities of multiphoton imaging. Here we discuss the current state of head-mounted multiphoton miniature microscopes and introduce a miniature head-mounted two-photon fiber-coupled microscope (2P-FCM) for neuronal imaging with active axial focusing enabled using a miniature electrowetting lens. The 2P-FCM enables three-dimensional two-photon optical recording of structure and activity at multiple focal planes in a freely moving mouse. Detailed methods are provided in this chapter on the 2P-FCM design, operation, and software for data analysis. |
| نوع الوثيقة: | book part |
| اللغة: | unknown |
| ردمك: | 978-1-07-162763-1 978-1-07-162764-8 1-07-162763-5 1-07-162764-3 |
| DOI: | 10.1007/978-1-0716-2764-8_7 |
| الإتاحة: | https://doi.org/10.1007/978-1-0716-2764-8_7Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.47A51673 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781071627631 9781071627648 1071627635 1071627643 |
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| DOI: | 10.1007/978-1-0716-2764-8_7 |