دورية أكاديمية
Interactions between glycopyrronium and indacaterol on cholinergic neurotransmission and contractile response in bovine trachealis
| العنوان: | Interactions between glycopyrronium and indacaterol on cholinergic neurotransmission and contractile response in bovine trachealis |
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| المؤلفون: | Baroffio M., Barisione G., Brusasco V. |
| المساهمون: | Baroffio, M., Barisione, G., Brusasco, V. |
| بيانات النشر: | BioMed Central Ltd. 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND |
| سنة النشر: | 2017 |
| المجموعة: | Università degli Studi di Genova: CINECA IRIS |
| مصطلحات موضوعية: | Acetylcholine, Airway smooth muscle, Long-acting muscarinic antagonist, Long-acting β-adrenoceptor agonist, Adrenergic beta-2 Receptor Agonist, Animal, Bronchodilator Agent, Cattle, Cholinergic Fiber, Dose-Response Relationship, Drug, Drug Interaction, Electric Stimulation, Glycopyrrolate, In Vitro Technique, Indan, Muscarinic Antagonist, Muscle Contraction, Muscle, Smooth, Quinolone, Synaptic Transmission, Trachea |
| الوصف: | Background: Muscarinic-receptor antagonists and β-adrenoceptor agonists are used, alone or in combination, as first-line treatment for chronic obstructive pulmonary disease. Both drugs decrease airway smooth muscle tone by post-junctional mechanisms but they may have opposing effects on pre-junctional acetylcholine (ACh)-release. Methods: We studied the effects of the muscarinic-receptor antagonist glycopyrronium (GLY), the β-adrenoceptor agonist indacaterol (IND) and their combination on electrically-induced ACh-release and contractile response in isolated bovine trachealis. Data were analyzed by paired t-test and analysis of variance for repeated or independent measures with Newmann-Keuls post-hoc test when appropriate. Results: GLY 10-8 M decreased contractile response by 19 ± 6% (p = 0.010) without altering ACh-release. GLY 10-7 M and 10-6 M almost abolished contractile responses even if the ACh-release was increased by 27 ± 19% (p < 0.001) and 20 ± 8% (p = 0.004), respectively. IND 10-7 M had no significant effects on contractile response and ACh-release, whereas IND 10-6 M reduced contractile response by 24 ± 12% (p = 0.002) without altering ACh-release. IND 10-5 M decreased contractile response by 51 ± 17% (p < 0.001) and ACh-release by 22 ± 11% (p = 0.004). Co-incubation with GLY 10-8 M and IND 10-7 M did not alter ACh-release but inhibited contractile response by 41 ± 8% (p < 0.001). The latter effect was greater than with GLY 10-8 M, or IND 10-7 M, or IND 10-6 M given separately (p < 0.001 for all). The increment of ACh-release caused by GLY was attenuated by IND 10-5 M, though this did not affect contractile response. Conclusions: At equimolar concentration, GLY alone attenuates airway smooth muscle contraction more than IND, despite an increased ACh-release. Combination of GLY with IND at submaximal concentrations has more than additive effect suggesting a synergistic post-junctional effect. Adding GLY to IND provides a greater inhibitory effect on airway smooth muscle contraction than ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28754132; info:eu-repo/semantics/altIdentifier/wos/WOS:000407041900001; volume:18; firstpage:145; lastpage:145; numberofpages:1; journal:RESPIRATORY RESEARCH; http://hdl.handle.net/11567/968787Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85026308458 |
| DOI: | 10.1186/s12931-017-0627-5 |
| الإتاحة: | https://doi.org/10.1186/s12931-017-0627-5Test http://hdl.handle.net/11567/968787Test |
| رقم الانضمام: | edsbas.46F2F60C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12931-017-0627-5 |
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