دورية أكاديمية
Sugarcane mild mosaic virus: The rediscovery of an identified but unrecognized virus
| العنوان: | Sugarcane mild mosaic virus: The rediscovery of an identified but unrecognized virus |
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| المؤلفون: | Mollov, Dimitre S., Filloux, Denis, Grinstead, Samuel, Bolus, Stephen, Claude, Lisa, Fernandez, Emmanuel, Julian, Charlotte, Candresse, Thierry, Lockhart, Benham E.L., Roumagnac, Philippe, Rott, Philippe, Daugrois, Jean-Heinrich |
| المصدر: | Plant Pathology |
| سنة النشر: | 2023 |
| المجموعة: | CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) |
| مصطلحات موضوعية: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Virus des végétaux, Virus mosaïque canne à sucre, Génome, Contrôle de maladies, Pathologie végétale, http://aims.fao.org/aos/agrovoc/c_5985Test, http://aims.fao.org/aos/agrovoc/c_35631Test, http://aims.fao.org/aos/agrovoc/c_3224Test, http://aims.fao.org/aos/agrovoc/c_2327Test, http://aims.fao.org/aos/agrovoc/c_5974Test |
| الوصف: | The complete genome sequence of a previously uncharacterized sugarcane-infecting virus of the genus Ampelovirus was obtained by high-throughput sequencing (HTS) and rapid amplification of cDNA ends. This genome consists of 13,114 nucleotides (nt) and harbours seven open reading frames. Phylogenetic and pairwise identity analyses with the complete or near-complete genome sequences of 16 isolates revealed that they all belong to a new Ampelovirus species, family Closteroviridae. These 16 isolates were distributed in three phylogenetic groups and potential recombinant events were identified for only one of these isolates. An immunocapture-reverse transcription-PCR (IC-RT-PCR) assay was developed with antibodies previously produced against a clostero-like virus that had not yet been recognized by the International Committee on Taxonomy of Viruses but for which the name sugarcane mild mosaic virus (SCMMV) had been proposed. The new ampelovirus was detected by IC-RT-PCR, and Sanger-sequenced amplicons confirmed the identity of the virus based on HTS data. SCMMV was detected in 65 of 350 samples of quarantined sugarcane germplasm using RT-PCR targeting the hsp70 gene. These samples originated from Africa, the Caribbean, North America, South America and South-east Asia, thus revealing that SCMMV is present in many sugarcane-growing regions of the world. The complete or near-complete sequences reported here represent the first genomic sequences for SCMMV. Furthermore, the newly developed RT-PCR diagnostic assay will facilitate the understanding of SCMMV epidemiology and will help improve management practices. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | http://agritrop.cirad.fr/602507Test/; Sugarcane mild mosaic virus: The rediscovery of an identified but unrecognized virus. Mollov Dimitre S., Filloux Denis, Grinstead Samuel, Bolus Stephen, Claude Lisa, Fernandez Emmanuel, Julian Charlotte, Candresse Thierry, Lockhart Benham E.L., Roumagnac Philippe, Rott Philippe, Daugrois Jean-Heinrich. 2023. Plant Pathology, 72 (2) : 312-321.https://doi.org/10.1111/ppa.13654Test; http://agritrop.cirad.fr/602507/7/602507.pdfTest |
| DOI: | 10.1111/ppa.13654 |
| الإتاحة: | https://doi.org/10.1111/ppa.13654Test http://agritrop.cirad.fr/602507Test/ http://agritrop.cirad.fr/602507/7/602507.pdfTest |
| حقوق: | Cirad license ; info:eu-repo/semantics/restrictedAccess ; https://agritrop.cirad.fr/mention_legale.htmlTest |
| رقم الانضمام: | edsbas.46B8A287 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/ppa.13654 |
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