دورية أكاديمية
Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice
| العنوان: | Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice |
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| المؤلفون: | Nanishi, Etsuro, Borriello, Francesco, Seo, Hyuk-Soo, O’Meara, Timothy R., McGrath, Marisa E., Saito, Yoshine, Chen, Jing, Diray-Arce, Joann, Song, Kijun, Xu, Andrew Z., Barman, Soumik, Menon, Manisha, Dong, Danica, Caradonna, Timothy M., Feldman, Jared, Hauser, Blake M., Schmidt, Aaron G., Baden, Lindsey R., Ernst, Robert K., Dillen, Carly, Yu, Jingyou, Chang, Aiquan, Hilgers, Luuk, Platenburg, Peter Paul, Dhe-Paganon, Sirano, Barouch, Dan H., Ozonoff, Al, Zanoni, Ivan, Frieman, Matthew B., Dowling, David J., Levy, Ofer |
| المساهمون: | U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases, Massachusetts Consortium on Pathogenesis Readiness, U.S. Department of Health & Human Services | National Institutes of Health, U.S. Department of Health & Human Services | Biomedical Advanced Research and Development Authority, United States Department of Defense | Defense Advanced Research Projects Agency |
| المصدر: | npj Vaccines ; volume 8, issue 1 ; ISSN 2059-0105 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Pharmacology (medical), Infectious Diseases, Pharmacology, Immunology |
| الوصف: | Development of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While demonstrating superior immunogenicity to soluble receptor-binding domain (RBD), RBD displayed as a protein nanoparticle (RBD-NP) generated limited antibody responses. Comparison of multiple adjuvants including AddaVax , AddaS03 , and AS01B in young and aged mice demonstrated that an oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMS:O/W) most effectively enhanced RBD-NP-induced cross-neutralizing antibodies and protection across age groups. CMS:O/W enhanced antigen retention in the draining lymph node, induced injection site, and lymph node cytokines, with CMS inducing MyD88-dependent Th1 cytokine polarization. Furthermore, CMS and O/W synergistically induced chemokine production from human PBMCs. Overall, CMS:O/W adjuvant may enhance immunogenicity and protection of vulnerable populations against SARS-CoV-2 and other infectious pathogens. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41541-023-00610-4 |
| الإتاحة: | https://doi.org/10.1038/s41541-023-00610-4Test https://www.nature.com/articles/s41541-023-00610-4.pdfTest https://www.nature.com/articles/s41541-023-00610-4Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.466DF495 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41541-023-00610-4 |
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