دورية أكاديمية
Host sequences flanking the human T-cell leukemia virus type 1 provirus in vivo.
| العنوان: | Host sequences flanking the human T-cell leukemia virus type 1 provirus in vivo. |
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| المؤلفون: | Leclercq, I, Mortreux, F, Cavrois, M, Leroy, A, Gessain, A, Wain-Hobson, S, Wattel, E |
| المساهمون: | Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur Paris (IP), Université Paris Diderot - Paris 7 (UPD7), Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire et approches thérapeutiques des hémopathies malignes, IRCL-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Biomolécules Max Mousseron Pôle Chimie Balard (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Epidémiologie et Physiopathologie des Virus Oncogènes (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur Paris (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Rétrovirologie Moléculaire, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by grants from the Association pour la Recherche sur le Cancer, from the Ligue Nationale contre le Cancer (Comité Pas de Calais), and from the Fondation Contre la Leucémie. I.L. and F.M. were supported by bursaries from the Ministère de l'Enseignement Supérieur et de la Recherche. We thank P. Wattre and collaborators, who kindly received us in their laboratories for DNA extraction, digestion, ligation, and PCR. We also thank Marie-Dominique Reynaud for assistance. |
| المصدر: | ISSN: 1090-9508 ; Journal of Human Virology ; https://hal.science/hal-00116159Test ; Journal of Human Virology, 2000, pp.2305-12. ⟨10.1128/JVI.74.5.2305-2312.2000⟩. |
| بيانات النشر: | HAL CCSD Lippincott Williams & Wilkins (LWW) |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | AT Rich Sequence, DNA/chemistry, DNA, Viral/genetics, HTLV-I Infections/genetics/*virology, Human T-lymphotropic virus 1/*genetics, Humans, Leukemia, T-Cell/genetics/virology, Proviruses/*genetics, Research Support, Non-U.S. Gov't, Sequence Homology, Nucleic Acid, Virus Integration, OCIS 000.1430, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | International audience ; Human pathogenic retroviruses do not have common loci of integration. However, many factors, such as chromatin structure, transcriptional activity, DNA-protein interaction, CpG methylation, and nucleotide composition of the target sequence, may influence integration site selection. These features have been investigated by in vitro integration reactions or by infection of cell lines with recombinant retroviruses. Less is known about target choice for integration in vivo. The present study was conducted in order to assess the characteristics of cellular sequences targeted for human T-cell leukemia virus type 1 (HTLV-1) integration in vivo. Sequencing integration sites from >/=200 proviruses (19 kb of sequence) isolated from 29 infected individuals revealed that HTLV-1 integration is not random at the level of the nucleotide sequence. The virus was found to integrate in A/T-rich regions with a weak consensus sequence at positions within and without of the hexameric repeat generated during integration. These features were not associated with a preference for integration near active regions or repeat elements of the host chromosomes. Most or all of the regions of the genome appear to be accessible to HTLV-1 integration. As with integration in vitro, integration specificity in vivo seems to be determined by local features rather than by the accessibility of specific regions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/10666261; hal-00116159; https://hal.science/hal-00116159Test; PUBMED: 10666261; PUBMEDCENTRAL: PMC111712 |
| DOI: | 10.1128/JVI.74.5.2305-2312.2000 |
| الإتاحة: | https://doi.org/10.1128/JVI.74.5.2305-2312.2000Test https://hal.science/hal-00116159Test |
| رقم الانضمام: | edsbas.45B5AEA9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JVI.74.5.2305-2312.2000 |
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