التفاصيل البيبلوغرافية
| العنوان: |
Histo-FISH protocol to detect bacterial compositions and biofilms formation in vivo |
| المؤلفون: |
Madar, M., Slizova, M., Czerwinski, J., Hrckova, G., Mudronova, D., Gancarcikova, S., Popper, M., Pistl, J., Soltys, J., Nemcova, R. |
| المصدر: |
Beneficial Microbes ; volume 6, issue 6, page 899-907 ; ISSN 1876-2883 1876-2891 |
| بيانات النشر: |
Wageningen Academic Publishers |
| سنة النشر: |
2015 |
| مصطلحات موضوعية: |
Microbiology (medical), Microbiology |
| الوصف: |
The study of biofilm function in vivo in various niches of the gastrointestinal tract (GIT) is rather limited. It is more frequently used in in vitro approaches, as an alternative to the studies focused on formation mechanisms and function of biofilms, which do not represent the actual in vivo complexity of microbial structures. Additionally, in vitro tests can sometimes lead to unreliable results. The goal of this study was to develop a simple approach to detect bacterial populations, particularly Lactobacillus and Bifidobacterium in biofilms, in vivo by the fluorescent in situ hybridisation (FISH) method. We standardised a new Histo-FISH method based on specific fluorochrome labelling probes which are able to detect Lactobacillus spp. and Bifidobacterium spp. within biofilms on the mucosal surface of the GIT embedded in paraffin in histological slices. This method is also suitable for visualisation of bacterial populations in the GIT internal content. Depending on the labelling probes, the Histo-FISH method has the potential to detect other probiotic strains or pathogenic bacteria. This original approach permits us to analyse bacterial colonisation processes as well as biofilm formation in stomach and caecum of BALB/c and germ-free mice. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.3920/bm2015.0016 |
| DOI: |
10.3920/BM2015.0016 |
| الإتاحة: |
https://doi.org/10.3920/bm2015.0016Test |
| رقم الانضمام: |
edsbas.44FB8A63 |
| قاعدة البيانات: |
BASE |
