دورية أكاديمية
Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography
| العنوان: | Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography |
|---|---|
| المؤلفون: | Oberthuer, Dominik, Knoška, Juraj, Mariani, Valerio, Tolstikova, Aleksandra, Adriano, Luigi, Awel, Salah, Barthelmess, Miriam, Dörner, Katerina, Xavier, P. Lourdu, Yefanov, Oleksandr, James, Daniel R., Nelson, Garrett, Wiedorn, Max O., Wang, Dingjie, Calvey, George, Chen, Yujie, Schmidt, Andrea, Szczepek, Michael, Frielingsdorf, Stefan, Lenz, Oliver, Snell, Edward, Robinson, Philip J., Šarler, Božidar, Beyerlein, Kenneth R., Belšak, Grega, Maček, Marjan, Wilde, Fabian, Aquila, Andrew, Boutet, Sébastien, Liang, Mengning, Hunter, Mark S., Scheerer, Patrick, Lipscomb, John D., Weierstall, Uwe, Bushnell, David A., Kornberg, Roger D., Spence, John C. H., Pollack, Lois, Chapman, Henry N., Bajt, Saša, Kovaleva, Elena G., Heymann, Michael, Gumprecht, Lars, Kirian, Richard A., Barty, Anton |
| المصدر: | Scientific reports 7, 46846 (2017). doi:10.1038/srep46846 |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2017 |
| المجموعة: | DESY Publication Database (PUBDB) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/000 |
| جغرافية الموضوع: | DE |
| الوصف: | Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000403843300001; info:eu-repo/semantics/altIdentifier/pmid/pmid:28635955; info:eu-repo/semantics/altIdentifier/issn/2045-2322; https://bib-pubdb1.desy.de/record/399213Test; https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2018-00518%22Test |
| الإتاحة: | https://doi.org/10.1038/srep46846Test https://doi.org/10.3204/PUBDB-2018-00518Test https://bib-pubdb1.desy.de/record/399213Test https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2018-00518%22Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.44E2D83F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |