التفاصيل البيبلوغرافية
| العنوان: |
Expression of Nkx2‐5‐GFP bacterial artificial chromosome transgenic mice closely resembles endogenous Nkx2‐5 gene activity |
| المؤلفون: |
Chi, Xuan, Zhang, Shu‐xing, Yu, Wei, DeMayo, Francesco J., Rosenberg, Susan M., Schwartz, Robert J. |
| المصدر: |
genesis ; volume 35, issue 4, page 220-226 ; ISSN 1526-954X 1526-968X |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2003 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Summary: Mouse Nkx2‐5 gene is essential for early heart development and it is regulated by a complex array of regulatory modules. In order to establish an efficient in vivo system for mapping the Nkx2‐5 genomic locus for regulatory regions, we developed improved homologous recombination technology for use in Escherichia coli and then knocked an IRES‐hrGFP reporter gene into Nkx2‐5 gene in a 120 kb Nkx2‐5 bacterial artificial chromosome (BAC) clone. We employed the recombination genes redα and redβ under the pBAD promoter, which was specifically induced by the addition of L‐arabinose. Recombination was selected for by our universal targeting cassette which conferred kanamycin resistance in bacterial cells and neomycin resistance in mammalian cells. Transgenic mouse lines generated from this modified BAC clone closely resembled the endogenous Nkx2‐5 expression in the heart, pylorus sphincter, and spleen, but expression was not detected in the tongue. Nkx2‐5 BAC‐GFP expression was copy number‐dependent and locus site‐independent. BAC transgenics using the GFP reporter offers an efficient model system to study gene expression and regulation. genesis 35:220–226, 2003. © 2003 Wiley‐Liss, Inc. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/gene.10181 |
| الإتاحة: |
https://doi.org/10.1002/gene.10181Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.44B7EB11 |
| قاعدة البيانات: |
BASE |
