دورية أكاديمية
Evolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003–2017
| العنوان: | Evolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003–2017 |
|---|---|
| المؤلفون: | Mwangi, Peter N., Page, Nicola Anne, Seheri, Mapaseka L., Mphahlele, M., Nadan, Sandrama, Esona, Mathew D., Kumwenda, Benjamin, Kamng’ona, Arox W., Donato, Celeste M., Steele, Duncan A., Ndze, Valantine N., Dennis, Francis E., Jere, Khuzwayo C., Nyaga, Martin M. |
| بيانات النشر: | Microbiology Society |
| سنة النشر: | 2022 |
| المجموعة: | University of Pretoria: UPSpace |
| مصطلحات موضوعية: | G2P[4] Group A rotavirus strains, Rotavirus, Sub-lineages, Whole-genome analysis |
| الوصف: | DATA STATEMENT : All supporting data, code and protocols have been provided within the article or through supplementary data files. ; The transient upsurge of G2P[4] group A rotavirus (RVA) after Rotarix vaccine introduction in several countries has been a matter of concern. To gain insight into the diversity and evolution of G2P[4] strains in South Africa pre- and post-RVA vaccination introduction, whole-genome sequencing was performed for RVA positive faecal specimens collected between 2003 and 2017 and samples previously sequenced were obtained from GenBank (n=103; 56 pre- and 47 post-vaccine). Pre-vaccine G2 sequences predominantly clustered within sub-lineage IVa-1. In contrast, post-vaccine G2 sequences clustered mainly within sub-lineage IVa-3, whereby a radical amino acid (AA) substitution, S15F, was observed between the two sub-lineages. Pre-vaccine P[4] sequences predominantly segregated within sub-lineage IVa while post-vaccine sequences clustered mostly within sub-lineage IVb, with a radical AA substitution R162G. Both S15F and R162G occurred outside recognised antigenic sites. The AA residue at position 15 is found within the signal sequence domain of Viral Protein 7 (VP7) involved in translocation of VP7 into endoplasmic reticulum during infection process. The 162 AA residue lies within the hemagglutination domain of Viral Protein 4 (VP4) engaged in interaction with sialic acid-containing structure during attachment to the target cell. Free energy change analysis on VP7 indicated accumulation of stable point mutations in both antigenic and non-antigenic regions. The segregation of South African G2P[4] strains into pre- and post-vaccination sub-lineages is likely due to erstwhile hypothesized stepwise lineage/sub-lineage evolution of G2P[4] strains rather than RVA vaccine introduction. Our findings reinforce the need for continuous whole-genome RVA surveillance and investigation of contribution of AA substitutions in understanding the dynamic G2P[4] epidemiology. ; The Bill and Melinda Gates ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
| اللغة: | English |
| تدمد: | 2057-5858 |
| العلاقة: | Mwangi, P.N., Page, N.A., Seheri, M.L. et al. 2022, 'Evolutionary changes between pre- and post-vaccine South African group A G2P[4] rotavirus strains, 2003–2017', Microbial Genomics, vol. 2022, no. 8, pp. 1-14. DOI 10.1099/mgen.0.000809.; http://hdl.handle.net/2263/92310Test |
| DOI: | 10.1099/mgen.0.000809 |
| الإتاحة: | https://doi.org/10.1099/mgen.0.000809Test http://hdl.handle.net/2263/92310Test |
| حقوق: | © 2022 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
| رقم الانضمام: | edsbas.43D666A4 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20575858 |
|---|---|
| DOI: | 10.1099/mgen.0.000809 |