دورية أكاديمية
Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M-3 receptor expression by INGAP-PP in insulin-producing cells
| العنوان: | Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M-3 receptor expression by INGAP-PP in insulin-producing cells |
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| المؤلفون: | Paula, Flavia M. M., Barbosa, Helena C. L., Carneiro, Everardo M., Persaud, Shanta J., Gagliardino, Juan J., Boschero, Antonio C., Souza, Kleber L. A. |
| المصدر: | Paula , F M M , Barbosa , H C L , Carneiro , E M , Persaud , S J , Gagliardino , J J , Boschero , A C & Souza , K L A 2010 , ' Requirement of NF-kappaB signalling pathway for modulation of the cholinergic muscarinic M-3 receptor expression by INGAP-PP in insulin-producing cells ' , European Journal of Pharmacology , vol. 642 , no. 1-3 , pp. 37 - 46 . https://doi.org/10.1016/j.ejphar.2010.05.056Test |
| سنة النشر: | 2010 |
| المجموعة: | King's College, London: Research Portal |
| الوصف: | The pentadecapeptide comprising the 104-118 amino acid sequence of the ilotropin-derived Reg3-related islet neogenesis-associated protein (INGAP-PP) has been implicated in beta cell neogenesis and enhancement of insulin secretion in pancreatic islets. The aim of this study was to investigate intracellular pathways by which INGAP-PP signals in insulin-producing cells. Treatment with INGAP-PP increased insulin secretion and intracellular calcium levels in MIN6 cells. INGAP-PP exposure activated c-Myc, serum and particularly nuclear factor-kappaB (NF-kappa B) response elements in insulin-producing cells (1.7 +/- 0.1, 1.8 +/- 0.1, 2.4 +/- 0.3 for RINm5F, and 1.3 +/- 0.1, 1.3 +/- 0.1 and 1.6 +/- 0.1 fold for MIN6 cells compared to controls, respectively). There was an increase in the proliferation rate of viable cells (162 +/- 17% for RINm5F and 155 13% for MIN6) that was accompanied by an increase in proliferating cell nuclear antigen (PCNA) protein expression (187 +/- 19% and 170 +/- 8% for RINm5F and MIN6 cells respectively) following INGAP-PP treatment. INGAP-PP increased the expression of the muscarinic M-3 receptor subtype (169 +/- 4% for RINm5F and 222 +/- 20% for MIN6 cells). Activation of multiple serum response elements by foetal calf serum also increased muscarinic M-3 receptor expression (173 +/- 9% for RINm5F and 140 +/- 7% for MIN6 cells). The blockade of NF-kappa B signalling pathway strongly decreased muscarinic M-3 receptor expression in response to both stimuli. In summary, a network of intracellular signals that includes activation of c-Myc signalling pathway and increased PCNA expression might be related to the increased proliferation rate of insulin-producing cells following incubation with INGAP-PP. NF-kappa B signalling plays an essential role in controlling the expression of the muscarinic M-3 receptor. (C) 2010 Elsevier B.V. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.ejphar.2010.05.056 |
| الإتاحة: | https://doi.org/10.1016/j.ejphar.2010.05.056Test https://kclpure.kcl.ac.uk/portal/en/publications/c8eec158-76e2-409c-9a6d-598815981091Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.42CD4A71 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ejphar.2010.05.056 |
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