التفاصيل البيبلوغرافية
| العنوان: |
Tandem Mass Tag-Based Quantitative Proteomics Reveals Implication of a Late Embryogenesis Abundant Protein (BnLEA57) in Seed Oil Accumulation in Brassica napus L. |
| المؤلفون: |
Zhou, Zhongjing, Lin, Baogang, Tan, Jinjuan, Hao, Pengfei, Hua, Shuijin, Deng, Zhiping |
| المساهمون: |
National Key Research and Development Program of China |
| المصدر: |
Frontiers in Plant Science ; volume 13 ; ISSN 1664-462X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2022 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| الوصف: |
Enhancing oil content is one of the major goals in Brassica napus breeding; however, genetic regulation of seed oil content in plants is complex and not fully elucidated. In this study, we report proteins that were differentially accumulated in immature seeds of 35 days after anthesis between two recombinant inbred lines with contrasting seed oil content, high oil content line (HOCL) and low oil content line (LOCL) using a multiplex isobaric tandem mass tags (TMT)-based quantitative proteomic approach. Over 4,600 proteins were quantified in seeds of the two lines, and 342 proteins showed differential accumulation between seeds of HOCL and LOCL. Gene Ontology enrichment analysis revealed that the differentially accumulated proteins were enriched in proteins involved in lipid biosynthesis and metabolism, photosynthesis, and nutrient reservoir activity. Western blot confirmed the increased abundance of a late embryogenesis abundant protein (BnLEA57) in HOCL seeds compared with LOCL seeds, and overexpression of either BnLEA57 gene or its homology BnLEA55 in transgenic Arabidopsis thaliana enhanced oil content in Arabidopsis seeds. Our work provides new insights into the molecular regulatory mechanism of seed oil content in B. napus . |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fpls.2022.907244 |
| DOI: |
10.3389/fpls.2022.907244/full |
| الإتاحة: |
https://doi.org/10.3389/fpls.2022.907244Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.42940E45 |
| قاعدة البيانات: |
BASE |
