دورية أكاديمية
Ancient Bacterial Class Alphaproteobacteria Cytochrome P450 Monooxygenases Can Be Found in Other Bacterial Species
| العنوان: | Ancient Bacterial Class Alphaproteobacteria Cytochrome P450 Monooxygenases Can Be Found in Other Bacterial Species |
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| المؤلفون: | Nzuza, Nomfundo, Padayachee, Tiara, Syed, Puleng Rosinah, Kryś, Justyna Dorota, Chen, Wanping, Gront, Dominik, Nelson, David R., Syed, Khajamohiddin |
| المساهمون: | Nzuza, Nomfundo, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, KwaDlangezwa 3886, South Africa, nomfundonzuza11@gmail.com, Padayachee, Tiara, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, KwaDlangezwa 3886, South Africa, teez07padayachee@gmail.com, Syed, Puleng Rosinah, Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa, prosinah@gmail.com, Kryś, Justyna Dorota, Faculty of Chemistry, Biological and Chemical Research Center, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland, juchxd@gmail.com, Chen, Wanping, Department of Molecular Microbiology and Genetics, University of Göttingen, 37077 Göttingen, Germany, chenwanping1@foxmail.com, Gront, Dominik, Faculty of Chemistry, Biological and Chemical Research Center, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland, dgront@gmail.com, Nelson, David R., Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA, drnelson1@gmail.com, Syed, Khajamohiddin, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, KwaDlangezwa 3886, South Africa, khajamohiddinsyed@gmail.com |
| سنة النشر: | 2021 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | Cytochrome P450 monooxygenases (CYPs/P450s), heme-thiolate proteins, are well-known players in the generation of chemicals valuable to humans and as a drug target against pathogens. Understanding the evolution of P450s in a bacterial population is gaining momentum. In this study, we report comprehensive analysis of P450s in the ancient group of the bacterial class Alphaproteobacteria. Genome data mining and annotation of P450s in 599 alphaproteobacterial species belonging to 164 genera revealed the presence of P450s in only 241 species belonging to 82 genera that are grouped into 143 P450 families and 214 P450 subfamilies, including 77 new P450 families. Alphaproteobacterial species have the highest average number of P450s compared to Firmicutes species and cyanobacterial species. The lowest percentage of alphaproteobacterial species P450s (2.4%) was found to be part of secondary metabolite biosynthetic gene clusters (BGCs), compared other bacterial species, indicating that during evolution large numbers of P450s became part of BGCs in other bacterial species. Our study identified that some of the P450 families found in alphaproteobacterial species were passed to other bacterial species. This is the first study to report on the identification of CYP125 P450, cholesterol and cholest-4-en-3-one hydroxylase in alphaproteobacterial species (Phenylobacterium zucineum) and to predict cholesterol side-chain oxidation capability (based on homolog proteins) by P. zucineum. ; Cytochrome P450 monooxygenases (CYPs/P450s), heme-thiolate proteins, are well-known players in the generation of chemicals valuable to humans and as a drug target against pathogens. Understanding the evolution of P450s in a bacterial population is gaining momentum. In this study, we report comprehensive analysis of P450s in the ancient group of the bacterial class Alphaproteobacteria. Genome data mining and annotation of P450s in 599 alphaproteobacterial species belonging to 164 genera revealed the presence of P450s in only 241 species belonging to ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 |
| العلاقة: | https://resolver.sub.uni-goettingen.de/purl?gro-2/87898Test; ijms22115542 |
| DOI: | 10.3390/ijms22115542 |
| الإتاحة: | https://doi.org/10.3390/ijms22115542Test https://resolver.sub.uni-goettingen.de/purl?gro-2/87898Test |
| حقوق: | CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.4226E4EB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms22115542 |