دورية أكاديمية
Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7
| العنوان: | Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7 |
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| المؤلفون: | Jakobs, Barbara D., Spannagel, Lisa, Purvanov, Vladimir, Uetz-von Allmen, Edith, Matti, Christoph, Legler, Daniel F. |
| المصدر: | International Journal of Molecular Sciences. 2019, 20(10), 2597. eISSN 1422-0067. Available under: doi:10.3390/ijms20102597 |
| سنة النشر: | 2019 |
| المجموعة: | University of Konstanz: Konstanz Online Publication Server (KOPS) |
| مصطلحات موضوعية: | chemokine receptor, CCR7, chemokines, CCL19, nanobodies, 2-adrenergic receptor, bimolecular fluorescence complementation, split-luciferase complementation, ddc:570 |
| الوصف: | The chemokine receptor CCR7 plays a pivotal role in health and disease. In particular, CCR7 controls homing of antigen-bearing dendritic cells and T cells to lymph nodes, where adaptive immune responses are initiated. However, CCR7 also guides T cells to inflamed synovium and thereby contributes to rheumatoid arthritis and promotes cancer cell migration and metastasis formation. Nanobodies have recently emerged as versatile tools to study G-protein-coupled receptor functions and are being tested in diagnostics and therapeutics. In this study, we designed a strategy to engineer novel nanobodies recognizing human CCR7. We generated a nanobody library based on a solved crystal structure of the nanobody Nb80 recognizing the β 2 -adrenergic receptor (β 2 AR) and by specifically randomizing two segments within complementarity determining region 1 (CDR1) and CDR3 of Nb80 known to interact with β 2 AR. We fused the nanobody library to one half of split-YFP in order to identify individual nanobody clones interacting with CCR7 fused to the other half of split-YFP using bimolecular fluorescence complementation. We present three novel nanobodies, termed Nb1, Nb5, and Nb38, that recognize human CCR7 without interfering with G-protein-coupling and downstream signaling. Moreover, we were able to follow CCR7 trafficking upon CCL19 triggering using Nb1, Nb5, and Nb38. ; published |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-66958-784-2 1-66958-784-3 |
| العلاقة: | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-13zy0347kj6w93Test; http://dx.doi.org/10.3390/ijms20102597Test |
| DOI: | 10.3390/ijms20102597 |
| الإتاحة: | https://doi.org/10.3390/ijms20102597Test http://nbn-resolving.de/urn:nbn:de:bsz:352-2-13zy0347kj6w93Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.41CBFD77 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781669587842 1669587843 |
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| DOI: | 10.3390/ijms20102597 |