دورية أكاديمية
AMBRA1 phosphorylation by CDK1 and PLK1 regulates mitotic spindle orientation
| العنوان: | AMBRA1 phosphorylation by CDK1 and PLK1 regulates mitotic spindle orientation |
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| المؤلفون: | Faienza, Fiorella, Polverino, Federica, Rajendraprasad, Girish, Milletti, Giacomo, Hu, Zehan, Colella, Barbara, Gargano, Deborah, Strappazzon, Flavie, Rizza, Salvatore, Vistesen, Mette Vixø, Luo, Yonglun, Antonioli, Manuela, Cianfanelli, Valentina, Ferraina, Caterina, Fimia, Gian Maria, Filomeni, Giuseppe, De Zio, Daniela, Dengjel, Joern, Barisic, Marin, Guarguaglini, Giulia, Di Bartolomeo, Sabrina, Cecconi, Francesco |
| المصدر: | Faienza , F , Polverino , F , Rajendraprasad , G , Milletti , G , Hu , Z , Colella , B , Gargano , D , Strappazzon , F , Rizza , S , Vistesen , M V , Luo , Y , Antonioli , M , Cianfanelli , V , Ferraina , C , Fimia , G M , Filomeni , G , De Zio , D , Dengjel , J , Barisic , M , Guarguaglini , G , Di Bartolomeo , S & Cecconi , F 2023 , ' AMBRA1 phosphorylation by CDK1 and .... |
| سنة النشر: | 2023 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | Humans, Phosphorylation, Protein Serine-Threonine Kinases/genetics, Spindle Apparatus/metabolism, Cell Cycle Proteins/metabolism, Mitosis, Cell Cycle, HeLa Cells, CDC2 Protein Kinase/metabolism, Adaptor Proteins, Signal Transducing/metabolism |
| الوصف: | AMBRA1 is a crucial factor for nervous system development, and its function has been mainly associated with autophagy. It has been also linked to cell proliferation control, through its ability to regulate c-Myc and D-type cyclins protein levels, thus regulating G1-S transition. However, it remains still unknown whether AMBRA1 is differentially regulated during the cell cycle, and if this pro-autophagy protein exerts a direct role in controlling mitosis too. Here we show that AMBRA1 is phosphorylated during mitosis on multiple sites by CDK1 and PLK1, two mitotic kinases. Moreover, we demonstrate that AMBRA1 phosphorylation at mitosis is required for a proper spindle function and orientation, driven by NUMA1 protein. Indeed, we show that the localization and/or dynamics of NUMA1 are strictly dependent on AMBRA1 presence, phosphorylation and binding ability. Since spindle orientation is critical for tissue morphogenesis and differentiation, our findings could account for an additional role of AMBRA1 in development and cancer ontogenesis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://pure.au.dk/portal/en/publications/0fe13f70-6fc6-4c57-9da9-cb56cdaad6d6Test |
| DOI: | 10.1007/s00018-023-04878-6 |
| الإتاحة: | https://doi.org/10.1007/s00018-023-04878-6Test https://pure.au.dk/portal/en/publications/0fe13f70-6fc6-4c57-9da9-cb56cdaad6d6Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.40F21B1B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00018-023-04878-6 |
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