دورية أكاديمية

Label-Free Optical Imaging of the Electron Transfer in Single Live Microbial Cells

التفاصيل البيبلوغرافية
العنوان: Label-Free Optical Imaging of the Electron Transfer in Single Live Microbial Cells
المؤلفون: Yi-Nan Liu (524291), Zhen-Ting Lv (10126053), Wen-Li Lv (10867294), Dong-Feng Liu (3945140), Xian-Wei Liu (1793137)
سنة النشر: 2023
مصطلحات موضوعية: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Biotechnology, Immunology, Infectious Diseases, Computational Biology, Space Science, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, unveiling ample heterogeneities, electrochemical activity parameters, plasmonic imaging technique, microbial cells using, noninvasive imaging method, free optical imaging, microbial cells, various biological, situ <, remains challenging, redox reaction, formal potential, electrons transferred, electron transfer, directly probe, chemical processes, cell level, c <
الوصف: Measurement of electron transfer at the single-particle or -cell level is crucial to the in situ study of basic chemical and biological processes. However, it remains challenging to directly probe the microbial extracellular electron transfer process due to the weakness of signals and the lack of techniques. Here, we present a label-free and noninvasive imaging method that is able to measure the electron transfer in microbial cells. We measured the extracellular electron transfer processes by imaging the redox reaction of c -type outer membrane cytochromes in microbial cells using a plasmonic imaging technique, and obtained the electrochemical activity parameters (formal potential and number of electrons transferred) of multiple individual microbial cells, allowing for unveiling ample heterogeneities in electron transfer at the single-cell level. We anticipate that this method will contribute to the study of electron transfer in various biological and chemical processes.
نوع الوثيقة: article in journal/newspaper
اللغة: unknown
العلاقة: https://figshare.com/articles/journal_contribution/Label-Free_Optical_Imaging_of_the_Electron_Transfer_in_Single_Live_Microbial_Cells/21806642Test
DOI: 10.1021/acs.nanolett.2c04018.s001
الإتاحة: https://doi.org/10.1021/acs.nanolett.2c04018.s001Test
حقوق: CC BY-NC 4.0
رقم الانضمام: edsbas.40A42C9A
قاعدة البيانات: BASE