مؤتمر
Influence of Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S rDNA
| العنوان: | Influence of Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S rDNA |
|---|---|
| المؤلفون: | Terrat, Sébastien, Christen, Richard, Dequiedt, Samuel, S., Mougel, Christophe, Lelievre, Mélanie, M., Maron, Pierre-Alain, Plassart, Pierre, Wincker, Patrick, Cruaud, Claudy, Jolivet, Claudy, C., Arrouays, Dominique, Bispo, Antonio, Lemanceau, Philippe, P., Ranjard, Lionel |
| المساهمون: | Agroécologie Dijon, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Biologie Virtuelle, Université Nice Sophia Antipolis (1965 - 2019) (UNS), Institut de Génomique d'Evry (IG), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), InfoSol (InfoSol), Institut National de la Recherche Agronomique (INRA), Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME) |
| المصدر: | NENUN ISME-Workshop: Extraction of Microbial DNA & RNA in Environmental Samples ; https://hal.science/hal-01208718Test ; NENUN ISME-Workshop: Extraction of Microbial DNA & RNA in Environmental Samples, Aug 2012, Copenhague, Denmark |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2012 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | pipeline, pyrosequencing, soil biodiversity, soil DNA, extraction, procedure, [SDV]Life Sciences [q-bio] |
| جغرافية الموضوع: | Copenhague, Denmark |
| الوصف: | Présentation orale faite lors de ce congrès EA SPE EcolDur GenoSol CT3 (vu ST) Présentation orale faite lors de ce congrèsEASPEEcolDurGenoSolCT3 (vu ST) ; During the last two decades, novel molecular and robust methods were developed that were well-suited to characterize soil microbial communities, as they provided access to previously hidden genetic resources. These methods were based essentially on soil DNA characterization and most efforts were devoted to optimize the soil DNA extraction procedure in order to obtain representative and suitable extracts for quantitative and qualitative characterization of microbial communities. Though several studies have evaluated the technical biases of these procedures to compare microbial abundance and community fingerprinting in diverse soils, the recent development of high throughput sequencing technology, which allow the scientific community to get hundreds of thousands of ribosomic sequences from a single metagenomic DNA, need to revisit our evaluation of soil DNA extraction efficiency to recover microbial diversity. Our study aimed at evaluating three soil DNA extraction procedures (two homemade protocols: Gns-GII and SY3, and one commercial kit: MOBIO) on seven contrasting soils for their efficiency: (i) in recovering soil DNA and, (ii) in the detection of bacterial species richness and evenness estimated with pyrosequencing of 16S rDNA. The seven studied soils originate from the soil library of the French Soil Quality Monitoring Network (RMQS), which contains more than 2,200 soils representative of the pedologic, climatic and land use conditions encountered on the French national territory. Soils were chosen because of their contrasting pH, texture, carbon organic content and land-use characteristics, known to strongly influence microbial abundance and diversity. Significant differences were systematically observed between procedures with, in certain soils, 10 times more DNA recovered than in the other protocols. In the same way, significant differences in the ... |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| العلاقة: | hal-01208718; https://hal.science/hal-01208718Test; https://hal.science/hal-01208718/documentTest; https://hal.science/hal-01208718/file/2012-123_1.pdfTest; PRODINRA: 271974 |
| الإتاحة: | https://hal.science/hal-01208718Test https://hal.science/hal-01208718/documentTest https://hal.science/hal-01208718/file/2012-123_1.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.3EFD7E54 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |