دورية أكاديمية
Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete
| العنوان: | Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete |
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| المؤلفون: | Wachter, Jenny, Cheff, Britney, Hillman, Chad, Carracoi, Valentina, Dorward, David W., Martens, Craig, Barbian, Kent, Nardone, Glenn, Renee Olano, L., Kinnersley, Margie, Secor, Patrick R., Rosa, Patricia A. |
| المصدر: | Nature Communications ; volume 14, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi . We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochetes in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18-depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS . The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-023-35897-3 |
| الإتاحة: | https://doi.org/10.1038/s41467-023-35897-3Test https://www.nature.com/articles/s41467-023-35897-3.pdfTest https://www.nature.com/articles/s41467-023-35897-3Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.3E97AA8B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-35897-3 |
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