دورية أكاديمية
No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia
| العنوان: | No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia |
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| المؤلفون: | Brogård Andersen, Hannah, Andersen, Mads, Bennedsgaard, Kristine Tang, Kerrn-Jespersen, Sigrid, Jacobsen Kyng, Kasper, Holm, Ida Elisabeth, Henriksen, Tine Brink |
| المصدر: | Brogård Andersen , H , Andersen , M , Bennedsgaard , K T , Kerrn-Jespersen , S , Jacobsen Kyng , K , Holm , I E & Henriksen , T B 2022 , ' No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia ' , Neuropediatrics , vol. 53 , no. 6 , pp. 423-431 . https://doi.org/10.1055/a-1889-8544Test |
| سنة النشر: | 2022 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | Hypoxia-ischemia, hypoxic-ischemic encephalopathy, immunohistochemistry, piglet model, remote ischemic postconditioning, Animals, Newborn, Vascular Endothelial Growth Factor A, Brain/pathology, Humans, Ischemia, Swine, Biomarkers, Hypoxia, Ischemic Postconditioning/methods, Disease Models, Animal |
| الوصف: | Background Despite therapeutic hypothermia, neonates with hypoxic-ischemic encephalopathy still develop neurological disabilities. We have previously investigated neuroprotection by remote ischemic postconditioning (RIPC) in newborn piglets following hypoxia-ischemia (HI). The aim of this study was to further investigate potential effects of RIPC on cerebral immunohistochemical markers related to edema, apoptosis, and angiogenesis. Methods Brain expression of aquaporin 4, caspase-3, B-cell lymphoma 2, and vascular endothelial growth factor was analyzed by immunohistochemistry in 23 piglets, randomly selected from a larger study of RIPC after HI. Twenty animals were subjected to 45 minutes of HI and randomized to treatment with and without RIPC, while three animals were randomized to sham procedures. RIPC was conducted by four conditioning cycles of 5-minute ischemia and reperfusion. Piglets were euthanized 72 hours after the HI insult. Results Piglets subjected to HI treated with and without RIPC were similar at baseline and following the HI insult. However, piglets randomized to HI alone had longer duration of low blood pressure during the insult. We found no differences in the brain expression of the immunohistochemical markers in any regions of interest or the whole brain between the two HI groups. Conclusion RIPC did not influence brain expression of markers related to edema, apoptosis, or angiogenesis in newborn piglets at 72 hours after HI. These results support previous findings of limited neuroprotective effect by this RIPC protocol. Our results may have been affected by the time of assessment, use of fentanyl as anesthetic, or limitations related to our immunohistochemical methods. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://pure.au.dk/portal/da/publications/no-differences-in-cerebral-immunohistochemical-markers-following-remote-ischemic-postconditioning-in-newborn-piglets-with-hypoxiaischemiaTest(2012f7bd-3892-4e1e-9b2b-dcd42acce038).html |
| DOI: | 10.1055/a-1889-8544 |
| الإتاحة: | https://doi.org/10.1055/a-1889-8544Test https://pure.au.dk/portal/da/publications/no-differences-in-cerebral-immunohistochemical-markers-following-remote-ischemic-postconditioning-in-newborn-piglets-with-hypoxiaischemiaTest(2012f7bd-3892-4e1e-9b2b-dcd42acce038).html https://pure.au.dk/ws/files/352044790/No_Differences_in_Cerebral_Immunohistochemical_Markers_following_Remote_Ischemic_Postconditioning_in_Newborn_Piglets_with_Hypoxia-Ischemia.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3DE1D7E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1055/a-1889-8544 |
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