دورية أكاديمية
Identification of bovine tuberculosis biomarkers to detect tuberculin skin test and IFNγ release assay false negative cattle
| العنوان: | Identification of bovine tuberculosis biomarkers to detect tuberculin skin test and IFNγ release assay false negative cattle |
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| المؤلفون: | Klepp, Laura Ines, Eirin, Maria Emilia, Garbaccio, Sergio Gabriel, Soria, Marcelo, Bigi, Fabiana, Blanco, Federico Carlos |
| بيانات النشر: | Elsevier |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | BIOMARKERS, BOVINE TUBERCULOSIS, DIAGNOSIS, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test |
| الوصف: | Bovine tuberculosis (bTB) is an important animal and zoonotic disease, which causes severe economic losses. The main focus of this study was to assess the predictive power of previously identified biomarkers of bTB in infected animals that were negative to the tuberculin skin test (TST). We studied 16 animals with bTB, in which the disease was confirmed by necropsy, and 16 healthy animals. The level of expression of ten biomarkers (CXCL9, THBS1, MMP9, IL-22, CXCL10, IFNγ, IL-17, FYVE, CD14, IL-1R) was evaluated by RT-qPCR upon stimulation or not of peripheral blood mononuclear cells with PPDb (purified protein derivative of bovine tuberculin). In this assay, CXCL9, THBS1, MMP9, IL-22 and IFNγ changed their expression level depending on the bTB status. In addition, we evaluated different biomarker candidates simultaneously to infer the animal condition. By performing an analysis with classification trees, we found that the sturdiest combination was IL-22, IFNγ and IL-1R. On the other hand, CXCL10, IFNγ and IL-22's expression distinguished between bTB positive animals that were negative to TST (TST false negative animals) and the bTB negative groups. Thus, these biomarkers are promising candidates to be tested as an ancillary diagnostic assay. In addition, the expression of CXCL10 and IL-22 exhibited also significant differences between the bTB positive animals that were undetectable by IFNγ release assay (IGRA) and TST tests (TST and IGRA false negative animals) and the bTB negative groups. Therefore, CXCL10 and IL-22 constitute candidate biomarkers that could complement the two most widely used diagnostic tests. ; Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina ; Fil: Eirin, Maria Emilia. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0034-5288 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0034528818303072Test; http://hdl.handle.net/11336/147887Test; Klepp, Laura Ines; Eirin, Maria Emilia; Garbaccio, Sergio Gabriel; Soria, Marcelo; Bigi, Fabiana; et al.; Identification of bovine tuberculosis biomarkers to detect tuberculin skin test and IFNγ release assay false negative cattle; Elsevier; Research in Veterinary Science; 122; 2-2019; 7-14; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.1016/j.rvsc.2018.10.016Test http://hdl.handle.net/11336/147887Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/arTest/ |
| رقم الانضمام: | edsbas.3D6D5CFF |
| قاعدة البيانات: | BASE |
| تدمد: | 00345288 |
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