دورية أكاديمية
Stereoselective in Vitro Formation of c-type Cytochrome Variants from Hydrogenobacter thermophilus Containing only a Single Thioether Bond
| العنوان: | Stereoselective in Vitro Formation of c-type Cytochrome Variants from Hydrogenobacter thermophilus Containing only a Single Thioether Bond |
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| المؤلفون: | Daltrop, Oliver, Smith, Kevin M., Ferguson, Stuart J. |
| المصدر: | Faculty Publications |
| بيانات النشر: | LSU Digital Commons |
| سنة النشر: | 2003 |
| المجموعة: | LSU Digital Commons (Louisiana State University) |
| الوصف: | In vitro formation of Hydrogenobacter thermophilus cytochrome c 552 has previously been demonstrated (Daltrop, O., Allen, J. W. A., Willis, A. C., and Ferguson, S. J. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 7872-7876). Now we report that the single cysteine variants of H. thermophilus c552, which bind heme via a single thioether bond, also form in vitro. Furthermore, reaction of the apocytochromes containing either AXXCH or CXXAH in the binding motif with 2-vinyldeuteroheme and 4-vinyldeuteroheme resulted predominantly in covalent attachment between Cys-11 and the 2-vinyl moiety and Cys-14 and the 4-vinyl functionality. This observation shows that the covalent attachment of heme to apocytochrome is stereoselective, indicating that the initial non-covalent complexes between apoprotein and heme have to be in the correct stereochemical orientation for preferential promotion of thioether bond formation. Additionally, the heme derivatives 2-vinyldeuteroheme and 4-vinyldeuteroheme were reacted with wild-type H. thermophilus c 552 to yield another modification of cytochromes containing only one thioether bond. These results show that the formation of the two thioether bonds in typical c-type cytochromes can occur independently from one another. Aspects of rotational isomerism of heme in heme-proteins are discussed. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.lsu.edu/chemistry_pubs/1611Test; https://digitalcommons.lsu.edu/context/chemistry_pubs/article/2612/viewcontent/1611.pdfTest |
| DOI: | 10.1074/jbc.M301967200 |
| الإتاحة: | https://doi.org/10.1074/jbc.M301967200Test https://digitalcommons.lsu.edu/chemistry_pubs/1611Test https://digitalcommons.lsu.edu/context/chemistry_pubs/article/2612/viewcontent/1611.pdfTest |
| رقم الانضمام: | edsbas.3BB0049C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1074/jbc.M301967200 |
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