التفاصيل البيبلوغرافية
| العنوان: |
Research on function and mechanisms of a novel small molecule WG449E for hypertrophic scar |
| المؤلفون: |
Shao, T., Tang, W., Li, Y., Gao, D., Lv, K., He, P., Song, Y., Gao, S., Liu, M., Chen, Y., Yi, Z. |
| المساهمون: |
National Natural Science Foundation of China |
| المصدر: |
Journal of the European Academy of Dermatology and Venereology ; volume 34, issue 3, page 608-618 ; ISSN 0926-9959 1468-3083 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2019 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Background Hypertrophic scars are complications of severe wound healing characterized by excessive fibrosis associated with aberrant function of fibroblasts. However, no available drugs can be utilized to effectively treat these scars. The transforming growth factor β ( TGF β) signalling pathway regulates collagen synthesis and plays an important role in scar formation. Objectives To evaluate the anti‐scar effects of TGF β inhibitors in vitro and in vivo . Methods Col1α2‐luciferase reporter assay was used to screen the compounds suppress type I collagen gene transcription. Sulforhodamine B colorimetric assay and colony formation assay were used to test the compound's effect on cell proliferation. Wound healing and transwell assay were performed to test the cell migration and invasion. Western blotting, immunofluorescence, immunohistochemistry and Q‐ PCR assay were used to determine the protein and mRNA levels. 3D cell contraction assay was used to examine the cell contraction. Flow cytometry was performed to analyse cell apoptosis. Masson stain, H&E stain and immunochemistry were used to analyse the scar formation in vivo . Results WG 449E, as one of the most potent inhibitors, was identified to significantly downregulate the mRNA and protein levels of collagen in hypertrophic scar‐derived fibroblasts through inhibiting Smad2/3 phosphorylation. WG 449E inhibited the proliferation, migration and contraction of fibroblasts in vitro and in vivo . In addition, WG 449E induced cell apoptosis through the activation of cleaved‐caspase3. Moreover, WG 449E significantly attenuated hypertrophic scar formation and collagen deposition in a mechanical load‐induced mouse model. Conclusions WG 449E is a potential candidate for the treatment of hypertrophic scars. WG 449E downregulates the mRNA and protein levels of collagen in hypertrophic scar‐derived fibroblasts through inhibiting Smad2/3 phosphorylation and nucleic localization. WG 449E blocks HSF migration and invasion by regulating F‐actin assignment. In ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/jdv.16028 |
| الإتاحة: |
https://doi.org/10.1111/jdv.16028Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.3B7C4576 |
| قاعدة البيانات: |
BASE |
