دورية أكاديمية
A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
| العنوان: | A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae |
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| المؤلفون: | Ogier, Jean-Claude, Duvic, Bernard, Lanois-Nouri, Anne, Givaudan, Alain, Gaudriault, Sophie |
| المساهمون: | Diversité, Génomes & Interactions Microorganismes - Insectes Montpellier (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM) |
| المصدر: | ISSN: 1932-6203. |
| بيانات النشر: | HAL CCSD Public Library of Science |
| سنة النشر: | 2016 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | insect nematodes, xenorhabdus, steinernema, nématode entomopathogène, arbre phylogénétique, entérobactérie, génomique des populations, [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
| الوصف: | Xenorhabdus is a bacterial symbiont of entomopathogenic Steinernema nematodes and is pathogenic for insects. Its life cycle involves a stage inside the insect cadaver, in which it competes for environmental resources with microorganisms from soil and the insect gut. Xenorhabdus is, thus, a useful model for identifying new interbacterial competition systems. For the first time, in an entomopathogenic bacterium, Xenorhabdus doucetiae strain FRM16, we identified a cdi-like locus. The cdi loci encode contact-dependent inhibition (CDI) systems composed of proteins from the two-partner secretion (TPS) family. CdiB is the outer membrane protein and CdiA is the toxic exoprotein. An immunity protein, CdiI, protects bacteria against inhibition. We describe here the growth inhibition effect of the toxic C-terminus of CdiA from X. doucetiae FRM16, CdiA-CTFRM16, following its production in closely and distantly related enterobacterial species. CdiA-CTFRM16 displayed Mg2+-dependent DNase activity, in vitro. CdiA-CT (FRM16)-mediated growth inhibition was specifically neutralized by CdiI(FRM16). Moreover, the cdi(FRM16) locus encodes an ortholog of toxin-activating proteins C that we named CdiC(FRM16). In addition to E. coli, the cdiBCAI-type locus was found to be widespread in environmental bacteria interacting with insects, plants, rhizospheres and soils. Phylogenetic tree comparisons for CdiB, CdiA and CdiC suggested that the genes encoding these proteins had co-evolved. By contrast, the considerable variability of CdiI protein sequences suggests that the cdiI gene is an independent evolutionary unit. These findings further characterize the sparsely described cdiBCAI-type locus. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-389482-0 0-00-389482-7 |
| العلاقة: | hal-01594494; https://hal.science/hal-01594494Test; https://hal.science/hal-01594494/documentTest; https://hal.science/hal-01594494/file/Ogier%202016%20PlosOne_%7B0CEAD97D-5A9E-4C95-9070-38D2ED489133%7D.pdfTest; PRODINRA: 384419; WOS: 000389482700159 |
| DOI: | 10.1371/journal.pone.0167443 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0167443Test https://hal.science/hal-01594494Test https://hal.science/hal-01594494/documentTest https://hal.science/hal-01594494/file/Ogier%202016%20PlosOne_%7B0CEAD97D-5A9E-4C95-9070-38D2ED489133%7D.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.3B3900C3 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780003894820 0003894827 |
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| DOI: | 10.1371/journal.pone.0167443 |