التفاصيل البيبلوغرافية
| العنوان: |
Methodological Note |
| المؤلفون: |
Evaluation Of Buccal, Lee Moore, John Wiencke, Clarence Eng, Shichun Zheng, Allan Smith |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://superfund.berkeley.edu/publications/./pdfs/01_moore_1.pdfTest. |
| سنة النشر: |
2001 |
| المجموعة: |
CiteSeerX |
| مصطلحات موضوعية: |
buccal cells, susceptibility, polymorphisms, molecular and genetic epidemiology |
| الوصف: |
This paper demonstrates that more DNA was collected per brush than per tongue depressor when the same DNA extraction procedure was used (686 ng versus 257 ng DNA per tongue depressor or brush; table 1). Although the number of cells collected was similar with a brush or tongue depressor, eight out of 12 brush samples contained over 500 ng of DNA. In contrast, the maximum yield for a tongue depressor was 510 ng/sample. When three brushes and three tongue depressors were used (table 2), brushes and tongue depressors still yielded about the same amount of DNA per sample as in table 1 (about 600 ng per brush and 250 ng per tongue depressor). Cytobrushes may yield more DNA because they remove healthier cells from deeper mucosal layers than tongue depressors. Only one out of 24 samples failed the PCR reaction. This sample was collected with a cytobrush and contained the least number of cells (80,000 cells/sample) and DNA (40 ng) |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.61.4524Test; http://superfund.berkeley.edu/publications/./pdfs/01_moore_1.pdfTest |
| الإتاحة: |
http://superfund.berkeley.edu/publications/./pdfs/01_moore_1.pdfTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.3AF1C648 |
| قاعدة البيانات: |
BASE |
