دورية أكاديمية
Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry
| العنوان: | Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry |
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| المساهمون: | Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı., orcid:0000-0001-5757-8450, orcid:0000-0003-3463-7483, Eyigör, Özhan, Minbay, Zehra, Çavuşoğlu, İlkin, ABE-5128-2020, ABC-1475-2020, 6603109907, 8220935200, 56009155800 |
| بيانات النشر: | Humana Press |
| سنة النشر: | 2010 |
| المجموعة: | Açık Erişim@BUU (Bursa Uludağ Üniversitesi) |
| مصطلحات موضوعية: | Prepro-orexin, Kainic acid, CNQX, Kainate, AMPA, Hypothalamus, Regulate feeding-behavior, Hypocretin orexin, Sleep, Neuroendocrine, Subunit, Systems, Expression, Peptides, Endocrinology & metabolism, 6-Cyano-7-nitroquinoxaline-2,3-dione, Animals, Excitatory amino acid agonists, Excitatory amino acid antagonists, Female, Immunohistochemistry, Intracellular signaling peptides and proteins, Neurons, Neuropeptides, Proto-oncogene proteins c-fos, Rats, sprague-dawley, Receptors, glutamate, Synaptic transmission |
| الوصف: | Orexin neuropeptides participate in the regulation of feeding as well as the regulation and maintenance of wakefulness and the cognitive functions. Orexin A and B share a common precursor, prepro-orexin and neurons are localized in the lateral hypothalamus. Physiological studies showed that these neurons are regulated by glutamatergic innervations. We aimed to assess the effects of kainic acid as a potent agonist for non-NMDA glutamate receptors in the activation of orexin neurons. We also analyzed the effect of glutamate antagonist CNQX, injected prior to kainic acid, on this activation. Expression of c-Fos protein was used as a marker for neuronal activation. Dual immunohistochemical labeling was performed for prepro-orexin and c-Fos and the percentages of c-Fos-expressing orexin neurons were obtained for control, kainic acid, and CNQX groups. Kainic acid injection caused statistically significant increase in the number of c-Fos-positive neurons when compared to control group (62.69 and 36.31%, respectively). Activation of orexin neurons was blocked, in part, by CNQX (43.36%). In the light of these results, it is concluded that glutamate takes part in the regulation of orexin neurons and partially exerts its effects through non-NMDA glutamate receptors and that orexin neurons express functional non-NMDA receptors. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0969-711X |
| العلاقة: | 104S286-SBAG-K-74; Makale - Uluslararası Hakemli Dergi; Endocrine; Eyigör, Ö. vd. (2010). "Activation of orexin neurons through non-NMDA glutamate receptors evidenced by c-Fos immunohistochemistry". Endocrine, 37(1), 167-172.; https://doi.org/10.1007/s12020-009-9284-xTest; https://link.springer.com/article/10.1007/s12020-009-9284-xTest; http://hdl.handle.net/11452/28809Test; 000273851800021; 2-s2.0-75949123104; 167; 172; 37 |
| DOI: | 10.1007/s12020-009-9284-x |
| الإتاحة: | https://doi.org/10.1007/s12020-009-9284-xTest http://hdl.handle.net/11452/28809Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.3A3DBC94 |
| قاعدة البيانات: | BASE |
| تدمد: | 0969711X |
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| DOI: | 10.1007/s12020-009-9284-x |