دورية أكاديمية
Targeting of non-coding RNAs encoded by novel MYC enhancers inhibits the proliferation of human hepatic carcinoma cells in vitro
| العنوان: | Targeting of non-coding RNAs encoded by novel MYC enhancers inhibits the proliferation of human hepatic carcinoma cells in vitro |
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| المؤلفون: | Choi, Hae In, An, Ga Yeong, Yoo, Eunyoung, Baek, Mina, Chai, Jin Choul, Binas, Bert, Lee, Young Seek, Jung, Kyoung Hwa, Chai, Young Gyu |
| المساهمون: | National Research Foundation of Korea |
| المصدر: | Scientific Reports ; volume 12, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | The proto-oncogene MYC is important for development and cell growth, however, its abnormal regulation causes cancer. Recent studies identified distinct enhancers of MYC in various cancers, but any MYC enhancer(s) in hepatocellular carcinoma (HCC) remain(s) elusive. By analyzing H3K27ac enrichment and enhancer RNA (eRNA) expression in cultured HCC cells, we identified six putative MYC enhancer regions. Amongst these, two highly active enhancers, located ~ 800 kb downstream of the MYC gene, were identified by qRT-PCR and reporter assays. We functionally confirmed these enhancers by demonstrating a significantly reduced MYC expression and cell proliferation upon CRISPR/Cas9-based deletion and/or antisense oligonucleotide (ASO)-mediated inhibition. In conclusion, we identified potential MYC enhancers of HCC and propose that the associated eRNAs may be suitable targets for HCC treatment. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-022-04869-w |
| الإتاحة: | https://doi.org/10.1038/s41598-022-04869-wTest https://www.nature.com/articles/s41598-022-04869-w.pdfTest https://www.nature.com/articles/s41598-022-04869-wTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.380B750E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-022-04869-w |
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