دورية أكاديمية
Biologically relevant murine models of chronic Pseudomonas aeruginosa respiratory infection
| العنوان: | Biologically relevant murine models of chronic Pseudomonas aeruginosa respiratory infection |
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| المؤلفون: | Rodgers, Aoife M., Lindsay, Jaime, Monahan, Avril, Dubois, Alice V., Faniyi, Aduragbemi A., Plant, Barry J., Mall, Marcus A., Ekkelenkamp, Miquel B., Elborn, Stuart, Ingram, Rebecca J. |
| المصدر: | Rodgers , A M , Lindsay , J , Monahan , A , Dubois , A V , Faniyi , A A , Plant , B J , Mall , M A , Ekkelenkamp , M B , Elborn , S & Ingram , R J 2023 , ' Biologically relevant murine models of chronic Pseudomonas aeruginosa respiratory infection ' , Pathogens , vol. 12 , no. 8 , 1053 . https://doi.org/10.3390/pathogens12081053Test |
| سنة النشر: | 2023 |
| المجموعة: | Queen's University Belfast: Research Portal |
| مصطلحات موضوعية: | General Immunology and Microbiology, Immunology and Allergy, Infectious Diseases, Microbiology (medical), Molecular Biology, /dk/atira/pure/subjectarea/asjc/2700/2723, name=Immunology and Allergy, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/2400/2400, name=General Immunology and Microbiology, /dk/atira/pure/subjectarea/asjc/2700/2726, name=Microbiology (medical), /dk/atira/pure/subjectarea/asjc/2700/2725, name=Infectious Diseases, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being |
| الوصف: | Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and the leading cause of infection in patients with cystic fibrosis (CF). The ability of P. aeruginosa to evade host responses and develop into chronic infection causes significant morbidity and mortality. Several mouse models have been developed to study chronic respiratory infections induced by P. aeruginosa, with the bead agar model being the most widely used. However, this model has several limitations, including the requirement for surgical procedures and high mortality rates. Herein, we describe novel and adapted biologically relevant models of chronic lung infection caused by P. aeruginosa. Three methods are described: a clinical isolate infection model, utilising isolates obtained from patients with CF; an incomplete antibiotic clearance model, leading to bacterial bounce-back; and the establishment of chronic infection; and an adapted water bottle chronic infection model. These models circumvent the requirement for a surgical procedure and, importantly, can be induced with clinical isolates of P. aeruginosa and in wild-type mice. We also demonstrate successful induction of chronic infection in the transgenic βENaC murine model of CF. We envisage that the models described will facilitate the investigations of host and microbial factors, and the efficacy of novel antimicrobials, during chronic P. aeruginosa respiratory infections. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://pure.qub.ac.uk/en/publications/c4e40b53-a2df-482c-86a4-27e5c46bc84dTest |
| DOI: | 10.3390/pathogens12081053 |
| الإتاحة: | https://doi.org/10.3390/pathogens12081053Test https://pure.qub.ac.uk/en/publications/c4e40b53-a2df-482c-86a4-27e5c46bc84dTest https://pureadmin.qub.ac.uk/ws/files/505383992/pathogens_12_01053.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.379594C |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/pathogens12081053 |
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