دورية أكاديمية
Identification of TP53 mutations in circulating tumour DNA in high grade serous ovarian carcinoma using next generation sequencing technologies
| العنوان: | Identification of TP53 mutations in circulating tumour DNA in high grade serous ovarian carcinoma using next generation sequencing technologies |
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| المؤلفون: | Calapre, Leslie, Giardina, Tindaro, Beasley, Aaron B., Reid, Anna L., Stewart, Colin, Amanuel, Benhur, Meniawy, Tarek M., Gray, Elin S. |
| المصدر: | Research outputs 2022 to 2026 |
| بيانات النشر: | Edith Cowan University, Research Online, Perth, Western Australia |
| سنة النشر: | 2023 |
| المجموعة: | Edith Cowan University (ECU, Australia): Research Online |
| مصطلحات موضوعية: | Plasma circulating tumour DNA, high grade serous ovarian carcinoma (HGSOC), cancer, Medical Specialties, Medicine and Health Sciences, Oncology |
| الوصف: | Plasma circulating tumour DNA (ctDNA) has been suggested to be a viable biomarker of response to treatment in patients with high grade serous ovarian carcinoma (HGSOC). TP53 mutations are present in more than 90 % of HGSOCs but somatic variants are distributed across all exonic regions of the gene, requiring next generation sequencing (NGS) technologies for mutational analysis. In this study, we compared the suitability of the Accel (Swift) and Oncomine (ThermoFisher) panels for identification of TP53 mutations in ctDNA of HGSOC patients (N = 10). Only 6 patients (60 %) were found to have TP53 mutations using the ACCEL panel but the addition of molecular tags in the Oncomine panel improved ctDNA detection with at least one mutation detected in all cases (100 %). Orthogonal validation of the 14 somatic variants found by Oncomine, using droplet digital PCR, confirmed 79 % (11/14) of the identified mutations. Overall, the Oncomine panel with unique molecular identifiers (UMI) appears more useful for ctDNA analysis in HGSOC. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://ro.ecu.edu.au/ecuworks2022-2026/1893Test; https://ro.ecu.edu.au/context/ecuworks2022-2026/article/2893/viewcontent/Identification_20of_20TP53_20mutations.pdfTest |
| DOI: | 10.1038/s41598-023-27445-2 |
| الإتاحة: | https://doi.org/10.1038/s41598-023-27445-2Test https://ro.ecu.edu.au/ecuworks2022-2026/1893Test https://ro.ecu.edu.au/context/ecuworks2022-2026/article/2893/viewcontent/Identification_20of_20TP53_20mutations.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.372FD42F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-023-27445-2 |
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