التفاصيل البيبلوغرافية
| العنوان: |
Structure of the human SAGA coactivator complex |
| المؤلفون: |
Herbst, Dominik A, Esbin, Meagan N, Louder, Robert K, Dugast-Darzacq, Claire, Dailey, Gina M, Fang, Qianglin, Darzacq, Xavier, Tjian, Robert, Nogales, Eva |
| المصدر: |
Nature Structural & Molecular Biology, vol 28, iss 12 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2021 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Biochemistry and Cell Biology, Bioinformatics and Computational Biology, Biological Sciences, Genetics, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Adaptor Proteins, Signal Transducing, Binding Sites, Cell Line, Tumor, Chromatin, Cryoelectron Microscopy, Gene Expression Regulation, HeLa Cells, Histone Acetyltransferases, Humans, Nuclear Proteins, Phytic Acid, Promoter Regions, Genetic, Protein Conformation, Regulatory Elements, Transcriptional, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Saccharomycetales, Transcription, Chemical Sciences |
| جغرافية الموضوع: |
989 - 996 |
| الوصف: |
The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification, DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been reported, there are noteworthy functional and compositional differences for this complex in metazoans. Here we present the cryogenic-electron microscopy (cryo-EM) structure of human SAGA (hSAGA) and show how the arrangement of distinct structural elements results in a globally divergent organization from that of yeast, with a different interface tethering the core module to the TRRAP subunit, resulting in a dramatically altered geometry of functional elements and with the integration of a metazoan-specific splicing module. Our hSAGA structure reveals the presence of an inositol hexakisphosphate (InsP6) binding site in TRRAP and an unusual property of its pseudo-(Ψ)PIKK. Finally, we map human disease mutations, thus providing the needed framework for structure-guided drug design of this important therapeutic target for human developmental diseases and cancer. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt8dp6q8p6; https://escholarship.org/uc/item/8dp6q8p6Test |
| الإتاحة: |
https://escholarship.org/uc/item/8dp6q8p6Test |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.371A9984 |
| قاعدة البيانات: |
BASE |
