التفاصيل البيبلوغرافية
| العنوان: |
Anti-inflammation of isoliquiritigenin via the inhibition of NF-κB and MAPK in LPS-stimulated MAC-T cells |
| المؤلفون: |
Li, Manman, Lu, Guicong, Ma, Xiao, Wang, Ruihong, Chen, Xihong, Yu, Yongxiong, Jiang, Caode |
| المصدر: |
BMC Veterinary Research ; volume 18, issue 1 ; ISSN 1746-6148 |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
General Veterinary, General Medicine |
| الوصف: |
Background The application of plant extracts has received great interest for the treatment of bovine mastitis. Isoliquiritigenin (ISL) is a rich dietary flavonoid that has significant antioxidative, anti-inflammatory and anticancer activities. This study was conducted to explore the protective efficacy and related mechanism of ISL against lipopolysaccharide (LPS)-stimulated oxidation and inflammation in bovine mammary epithelial cells (MAC-T) by in vitro experiments. Results Real-time PCR and ELISA assays indicated that ISL treatment at 2.5, 5 and 10 μg/mL significantly reduced the mRNA and protein expression of the oxidative indicators cyclooxygenase-2 and inducible nitric oxide synthase ( P < 0.01), and of the inflammatory cytokines interleukin-6 ( P < 0.05), interleukin-1β ( P < 0.01) and tumor necrosis factor-α ( P < 0.01) in LPS-stimulated MAC-T cells. Moreover, Western blotting and immunofluorescence tests indicated that the phosphorylation levels of nuclear factor kappa (NF-κB) p65 and the inhibitor of NF-κB were significantly decreased by ISL treatment, thus blocking the nuclear transfer of NF-κB p65. In addition, ISL attenuated the phosphorylation levels of p38, extracellular signal-regulated kinase and c-jun NH2 terminal kinase. Conclusions Our data demonstrated that ISL downregulated the LPS-induced inflammatory response in MAC-T cells. The anti-inflammatory and antioxidative activity of ISL involves the NF-κB and MAPK cascades. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1186/s12917-022-03414-1 |
| DOI: |
10.1186/s12917-022-03414-1.pdf |
| DOI: |
10.1186/s12917-022-03414-1/fulltext.html |
| الإتاحة: |
https://doi.org/10.1186/s12917-022-03414-1Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: |
edsbas.36C87953 |
| قاعدة البيانات: |
BASE |
