دورية أكاديمية
A mesofluidic multiplex immunosensor for detection of circulating cytokeratin-positive cells in the blood of breast cancer patients
| العنوان: | A mesofluidic multiplex immunosensor for detection of circulating cytokeratin-positive cells in the blood of breast cancer patients |
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| المؤلفون: | Breton, François, Bennetau, Bernard, Lidereau, Rosette, Thomas, Laurent, Regnier, Gilles, Ehrhart, Jean-Claude, Tauc, Patrick, Tran, Phuong-Lan |
| المساهمون: | Institut d'Alembert (IDA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie organique et organométallique (LCOO), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Genetique Moleculaire des Cancers d'Origine Epitheliale, Institut Curie Paris -Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Procédés et Ingénierie en Mécanique et Matériaux (PIMM), Conservatoire National des Arts et Métiers CNAM (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Stabilité Génétique et Oncogenèse (UMR 8200), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA) |
| المصدر: | ISSN: 1387-2176. |
| بيانات النشر: | HAL CCSD Springer Verlag |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Breast cancer, Circulating tumor cells (CTCs), Targeted cell capture, Immunosensor, Multiplex device, Long alkylsilane monolayer, [SPI.MAT]Engineering Sciences [physics]/Materials |
| الوصف: | International audience ; We have recently reported the analytical performance of an immunosensor comprising one mm-scale parallel plate laminar flow chamber and applied to capture MCF7 breast cancer cells (Ehrhart et al., Biosens. Bioelectr. 24, 467, 2008). Herein we present a new multiplex immunosensor embodying four parallel plate laminar flow chambers that fit onto a standard, functionalized, microscopy glass slide. The four surfaces are coated with long alkyl chain spacers of 21-aminohenicosyl trichlorosilane (AHTS) and then grafted with a monoclonal anti-human epithelial cell adhesion molecule (EpCAM) antibody specific of target cells to immobilize. We first demonstrate a significantly (P < 0.01) improved capacity of each of the four flow chambers of the multiplex immunosensor to capture MCF7 cells compared to the previous single chamber device. Second, in addition to an increase of cell immobilization, the multiplex device offers a versatile tool easily grafted with various purified antibodies onto the four surfaces. Third, we obtained high cell capture rate and efficiency of various numbers of MCF7 cells spiked in buffer containing an equal number of background leukocytes. And fourth, we demonstrate isolation efficiency of circulating tumor cells (CTCs) from peripheral blood drawn from a small cohort of patients with localized or metastatic breast cancer. This new multiplex immunosensor could be tested for its potential to capture different subpopulations of CTCs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02428520; https://hal.science/hal-02428520Test; https://hal.science/hal-02428520/documentTest; https://hal.science/hal-02428520/file/PIMM_BM_2011_REGNIER.pdfTest |
| DOI: | 10.1007/s10544-010-9465-7 |
| الإتاحة: | https://doi.org/10.1007/s10544-010-9465-7Test https://hal.science/hal-02428520Test https://hal.science/hal-02428520/documentTest https://hal.science/hal-02428520/file/PIMM_BM_2011_REGNIER.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.366CE88C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s10544-010-9465-7 |
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