دورية أكاديمية
A Nucleosome Interaction Module Is Required for Normal Function of Arabidopsis thaliana BRAHMA
| العنوان: | A Nucleosome Interaction Module Is Required for Normal Function of Arabidopsis thaliana BRAHMA |
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| المؤلفون: | Farrona, Sara, Hurtado, Lidia, Reyes, José C. |
| المساهمون: | Ministerio de Educación y Ciencia (España), Junta de Andalucía |
| بيانات النشر: | Academic Press Elsevier |
| سنة النشر: | 2007 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Chromatin, Bromodomain, swi/snf complex, AT-hook, Seed development |
| الوصف: | The BRAHMA (BRM) gene encodes the SNF2-type ATPase of the putative Arabidopsis thaliana SWI/SNF chromatin remodelling complex. This family of ATPases is characterized by the presence of a conserved catalytic domain and an arrangement of auxiliary domains, whose functions in the remodelling activity remains unclear. Here, we characterize, at the molecular and functional level, the carboxy-terminal part of Arabidopsis BRM. We have found three DNA-binding regions that bind various free DNA and nucleosomal probes with different specificity. One of these regions contains an AT-hook motif. The carboxy terminus also contains a bromodomain able to bind histones H3 and H4. We propose that this array of domains constitute a nucleosome interaction module that helps BRM to interact with its substrate. We also characterize an Arabidopsis mutant that expresses a BRM protein lacking the last 454 amino acid residues (BRM-ΔC), encompassing the bromodomain and two of the three DNA-binding activities identified. This mutant displays an intermediate phenotype between those of the wild-type and a null allele mutant, suggesting that the nucleosome interaction module is required for the normal function of BRM but it is not essential for the remodelling activity of BRM-containing SWI/SNF complexes. ; S. F. and L. H. were recipients of fellowships from the Spanish Ministerio de Educación y Ciencia. This work was supported by Ministerio de Educación y Ciencia (grants BMC2002–03198, BFU2005-01047) and by Junta de Andalucía (group CV1-284). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0022-2836 |
| العلاقة: | http://dx.doi.org/10.1016/j.jmb.2007.07.012Test; Sí; Journal of Molecular Biology 373(2): 240-250 (2007); http://hdl.handle.net/10261/270449Test; http://dx.doi.org/10.13039/501100011011Test |
| DOI: | 10.1016/j.jmb.2007.07.012 |
| DOI: | 10.13039/501100011011 |
| الإتاحة: | https://doi.org/10.1016/j.jmb.2007.07.012Test https://doi.org/10.13039/501100011011Test http://hdl.handle.net/10261/270449Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.362914B9 |
| قاعدة البيانات: | BASE |
| تدمد: | 00222836 |
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| DOI: | 10.1016/j.jmb.2007.07.012 |