دورية أكاديمية
Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats
| العنوان: | Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats |
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| المؤلفون: | Wang, Ya-Nan, Wang, Bo, Liang, Ming, Han, Cai-Yan, Zhang, Bin, Cai, Jie, Sun, Wei, Xing, Guo-Gang |
| المساهمون: | Xing, GG (reprint author), Peking Univ, Neurosci Res Inst, 38 Xue Yuan Rd, Beijing 100191, Peoples R China., Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China., Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China., Shandong Univ Chinese Med, Affiliated Hosp 2, Reprod Med Ctr, Jinan, Peoples R China., Minist Educ, Key Lab Neurosci, Beijing, Peoples R China., Minist Publ Hlth, Beijing, Peoples R China., Peking Univ, Neurosci Res Inst, 38 Xue Yuan Rd, Beijing 100191, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | 生育与不孕 |
| سنة النشر: | 2013 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | Asthenozoospermia, male infertility, CatSper1 channel, cyclophosphamide, Sheng-Jing-San recipe, MALE-FERTILITY, SEMINAL PLASMA, HYPERACTIVATED MOTILITY, POSTNATAL-DEVELOPMENT, GENE-EXPRESSION, MOUSE TESTIS, NULL SPERM, CA2+ ENTRY, INFERTILITY |
| الوصف: | Objective: To determine the expression of CatSper1 channel in epididymal spermatozoa in a rat model of asthenozoospermia, induced by cyclophosphamide (CP), and further examine the effects of soluble granules of Sheng-Jing-San (SJS), a traditional Chinese medicine recipe, on CatSper1 expression and sperm motility in the CP-induced asthenozoospermic rats. Design: Placebo-controlled, randomized trial. Setting: Neuroscience Research Institute, Peking University, China. Animal(s): Sexually mature male Sprague-Dawley rats (n = 60). Intervention(s): In the CP group, CP at the dose of 35 mg/kg intraperitoneally injected into rats once a day for 7 days; in the normal saline (NS) group, 0.9% saline solution was injected as control. Main Outcome Measure(s): Sperm motility and count were evaluated by computer-assisted sperm assay (CASA); protein and mRNA expression of CatSper1 channel in epididymal spermatozoa was determined by Western blotting and quantitative real-time RT-PCR, respectively. Result(s): The rats were randomly divided into five groups with 12 rats in each group: CP, normal saline (NS), CP + SJS, CP + NS, and treatment naive. In the CP + SJS group, after the last injection of CP, SJS at a dose of 30 mg/kg was intragastrically administrated to rats once a day for 14 days; in CP + NS group, saline solution instead of SJS was administrated as control. In the treatment naive group, rats were normally fed for 21 days as controls. We found a statistically significant reduction of the CatSper1 channel, which is associated with an impairment of sperm motility in the epididymal spermatozoa of CP-induced asthenozoospermic rats. Soluble granules of SJS could dramatically restore the CP-induced down-regulation of CatSper1 in epididymal spermatozoa, which greatly improved the sperm motility in the asthenozoospermic rats. Conclusion(s): Down-regulation of the CatSper1 channel in epididymal spermatozoa likely contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by SJS improves sperm ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0015-0282 |
| العلاقة: | FERTILITY AND STERILITY.2013,99,(2),579-587.; 657580; http://hdl.handle.net/20.500.11897/190713Test; WOS:000314662400049 |
| DOI: | 10.1016/j.fertnstert.2012.10.030 |
| الإتاحة: | https://doi.org/20.500.11897/190713Test https://doi.org/10.1016/j.fertnstert.2012.10.030Test https://hdl.handle.net/20.500.11897/190713Test |
| رقم الانضمام: | edsbas.34F1C557 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00150282 |
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| DOI: | 10.1016/j.fertnstert.2012.10.030 |