دورية أكاديمية
Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity.
| العنوان: | Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity. |
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| المؤلفون: | Szczesna, Magdalena, Huang, Yizhou, Lacoursiere, Rachel E, Bonini, Francesca, Pol, Vito, Koc, Fulya, Ward, Beatrice, Geurink, Paul P, Pruneda, Jonathan N, Thurston, Teresa L M |
| المصدر: | Cell Host Microbe ; ISSN:1934-6069 ; Volume:32 ; Issue:6 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Autophagy, Burkholderia, RNF213, TssM, Ubiquitin esterase, bacterial effector, cell-autonomous immunity, non-canonical ubiquitylation |
| الوصف: | Aspects of how Burkholderia escape the host's intrinsic immune response to replicate in the cell cytosol remain enigmatic. Here, we show that Burkholderia has evolved two mechanisms to block the activity of Ring finger protein 213 (RNF213)-mediated non-canonical ubiquitylation of bacterial lipopolysaccharide (LPS), thereby preventing the initiation of antibacterial autophagy. First, Burkholderia's polysaccharide capsule blocks RNF213 association with bacteria and second, the Burkholderia deubiquitylase (DUB), TssM, directly reverses the activity of RNF213 through a previously unrecognized esterase activity. Structural analysis provides insight into the molecular basis of TssM esterase activity, allowing it to be uncoupled from its isopeptidase function. Furthermore, a putative TssM homolog also displays esterase activity and removes ubiquitin from LPS, establishing this as a virulence mechanism. Of note, we also find that additional immune-evasion mechanisms exist, revealing that overcoming this arm of the host's immune response is critical to the pathogen. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.chom.2024.04.012Test; https://pubmed.ncbi.nlm.nih.gov/38870903Test |
| DOI: | 10.1016/j.chom.2024.04.012 |
| الإتاحة: | https://doi.org/10.1016/j.chom.2024.04.012Test https://pubmed.ncbi.nlm.nih.gov/38870903Test |
| حقوق: | Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. |
| رقم الانضمام: | edsbas.34DA10C8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.chom.2024.04.012 |
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