دورية أكاديمية
Polyclonal antibodies against potato spindle tuber viroid RNA
| العنوان: | Polyclonal antibodies against potato spindle tuber viroid RNA |
|---|---|
| المؤلفون: | Yuri Drygin, Tatiana Gasanova, Konstantin Butenko |
| المصدر: | Frontiers in Bioscience-Elite, Vol 14, Iss 1, p 7 (2022) |
| بيانات النشر: | IMR Press |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | potato spindle tuber viroid, dot-elisa, immune northern blotting, diagnostics, nucleic acid vaccine, Environmental sciences, GE1-350, Microbiology, QR1-502 |
| الوصف: | Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with synthesized conjugate viroid RNA-lysozyme. Analyses of polyclonal mouse and rabbit antisera as well as estimates of antibody specificity were performed by dot-Enzyme Linked Immunosorbent Assay (ELISA), sandwich ELISA, and northern immunoblotting. Antiserum obtained showed strong cross-reactions with cellular RNA. The viroid polyclonal antibody cross-reactions with cellular RNAs were depleted via titration antibodies by the plant cellular or commercial yeast RNA. We successfully used antibodies against the viroid RNA-lysozyme antigen to detect the wild-type potato viroid and diagnose potato viroid infection. We presume that intrinsic cross-reactions of RNA antibodies are potentially dangerous after nucleic acid vaccination. Research into the specificity of antibodies against viral RNAs is underway. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1945-0494 |
| العلاقة: | https://www.imrpress.com/journal/FBE/14/1/10.31083/j.fbe1401007Test; https://doaj.org/toc/1945-0494Test; https://doaj.org/article/69dfc960279f48c3b8004323e544287cTest |
| DOI: | 10.31083/j.fbe1401007 |
| الإتاحة: | https://doi.org/10.31083/j.fbe1401007Test https://doaj.org/article/69dfc960279f48c3b8004323e544287cTest |
| رقم الانضمام: | edsbas.34AB1662 |
| قاعدة البيانات: | BASE |
| تدمد: | 19450494 |
|---|---|
| DOI: | 10.31083/j.fbe1401007 |