دورية أكاديمية
Assessment and Molecular Docking of SARS-CoV-2 NSP3 and NSP12 Mutants in Iranian Patients in Golestan Province
| العنوان: | Assessment and Molecular Docking of SARS-CoV-2 NSP3 and NSP12 Mutants in Iranian Patients in Golestan Province |
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| المؤلفون: | Emad Behboudi, Alijan Tabarraei, Alireza Tahamtan, Mohammad Reza Kalani, Abdolvahab Moradi |
| المصدر: | International Journal of Pediatrics, Vol 10, Iss 7, Pp 16370-16380 (2022) |
| بيانات النشر: | Mashhad University of Medical Sciences |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | nsp3, nsp12, mutation, covid-19, golestan, Pediatrics, RJ1-570 |
| الوصف: | Background: Molecular analysis of SARS-CoV-2 genome is important to predict viral pathogenicity. In addition to transmission, replication is a key factor in pathogenicity of the virus. Notably, mutations in non-structural proteins (NSP3 and NSP12) can affect host immune response and viral replication. Therefore, this study was conducted to investigate different mutations of SARS-CoV-2 NSP3, and NSP12 during different waves of COVID-19 infection.Methods: We recruited 57 NGS sequences including 8 NGS sequences from Golestan SARS-CoV-2 RNA samples, obtained as part of clinical testing in different referral centers of Iran. After obtaining sequences from the global initiative on sharing all influenza data (GISAID), and evaluating and processing data, all sequences were aligned to the Wuhan variant genome (NC_045512.2) using MEGA6. The HDOCK server was used for molecular docking.Results: In NSP3, mutations in positions (nts 315, 545, 2666, 3264) were more frequent and among them mutation in positions including nt 545 (aa182) and nt 2666 (aa889) were associated with an increase in codon usage. In the term of NSP12, mutations in positions such as nts 406 (aa137), 965 (aa323), 1233, 1653, 1836, 2733 were more frequent. The molecular docking results showed more affinity in some variants of NSP3 and NSP12 as well.Conclusion: This study has assessed mutation in SARS-CoV-2 Nsp3, and NSP12 which are viral protease, and viral polymerase (RdRp). The mutations reported in this study may help this virus to replicate faster and evade the pharmaceutical agents which target viral polymerase activity and be very effective in viral pathogenesis. In addition, this study highlights the importance of ongoing genomic variation studies to be performed on SARS-CoV-2 variants. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2345-5047 2345-5055 |
| العلاقة: | https://ijp.mums.ac.ir/article_19957_0fb2a22b80355cedbb6e60bffdd6dbde.pdfTest; https://doaj.org/toc/2345-5047Test; https://doaj.org/toc/2345-5055Test; https://doaj.org/article/ec937446a7ff411bb07e965e509e934fTest |
| DOI: | 10.22038/ijp.2022.64007.4862 |
| الإتاحة: | https://doi.org/10.22038/ijp.2022.64007.4862Test https://doaj.org/article/ec937446a7ff411bb07e965e509e934fTest |
| رقم الانضمام: | edsbas.34708360 |
| قاعدة البيانات: | BASE |
| تدمد: | 23455047 23455055 |
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| DOI: | 10.22038/ijp.2022.64007.4862 |