دورية أكاديمية
Acidic and basic self-assembling peptide and peptide-graphene oxide hydrogels: characterisation and effect on encapsulated nucleus pulposus cells
| العنوان: | Acidic and basic self-assembling peptide and peptide-graphene oxide hydrogels: characterisation and effect on encapsulated nucleus pulposus cells |
|---|---|
| المؤلفون: | Ligorio, Cosimo, Vijayaraghavan, Aravind, Hoyland, Judith A., Saiani, Alberto |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | University of Nottingham: Repository@Nottingham |
| مصطلحات موضوعية: | pH effect, Peptide hydrogels, 3D cell culture, Nucleus pulposus |
| الوصف: | Extracellular pH can have a profound effect on cell metabolism, gene and protein expression. Nucleus pulposus (NP) cells, for example, under acidic conditions accelerate the production of degradative enzymes and pro-inflammatory cytokines, leading ultimately to intervertebral disc degeneration, a major cause of back pain. Self-assembling peptide hydrogels constitute a well-established class of biomaterials that could be exploited as pH-tunable platform to investigate cell behaviour under normal and non-physiological pH. In this paper we formulated acidic (pH = 4) and basic (pH = 9) hydrogels, from the same octapeptide FEFKFEFK (F8) (F = phenyalanine, E = glutamic acid, K = lysine), to test the effect of non-physiological pH on encapsulated NP cells. Similarly, graphene oxide-containing F8 hydrogels (GO-F8) were formulated as stiffer analogues. Acidic and basic hydrogels showed peculiar morphologies and rheological properties, with all systems able to buffer within 30 minutes of exposure to cell culture media. NP cells seeded in acidic F8 hydrogels showed a more catabolic phenotype compared to basic hydrogels, with increased gene expression of degradative enzymes (MMP-3, ADAMTS-4), neurotrophic factors (NGF and BDNF) and NF-κB p65 phosphorylation. Acidic GO-F8 hydrogels also induced a catabolic response, although milder than basic counterparts and with the highest gene expression of characteristic NP-matrix components, aggrecan and collagen II. In all systems, the cellular response had a peak within 3 days of encapsulation, thereafter decreasing over 7 days, suggesting a ‘transitory’ effect of hydrogel pH on encapsulated cells. This work gives an insight on the effect of pH (and pH buffering) on encapsulated NP cells and offers new designs of low and high pH peptide hydrogels for 3D cell culture studies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://nottingham-repository.worktribe.com/output/27854514Test; Acta Biomaterialia; Volume 143; Pagination 145-158; https://nottingham-repository.worktribe.com/file/27854514/1/Advance%20%20Chk%20Titlecnt%20%20%20Ne%20Acidic%20And%20Basic%20Self-assembling%20Peptide%20And%20Peptide-graphene%20Oxide%20Hydrogels%20%20Characterisation%20And%20Effect%20On%20Encapsulated%20Nucleus%20Pulposus%20Cells%20Global%20%20Chk%20Titlecnt%20%20%20ZTest |
| DOI: | 10.1016/j.actbio.2022.02.022 |
| الإتاحة: | https://doi.org/10.1016/j.actbio.2022.02.022Test https://nottingham-repository.worktribe.com/file/27854514/1/Advance%20%20Chk%20Titlecnt%20%20%20Ne%20Acidic%20And%20Basic%20Self-assembling%20Peptide%20And%20Peptide-graphene%20Oxide%20Hydrogels%20%20Characterisation%20And%20Effect%20On%20Encapsulated%20Nucleus%20Pulposus%20Cells%20Global%20%20Chk%20Titlecnt%20%20%20ZTest https://nottingham-repository.worktribe.com/output/27854514Test |
| حقوق: | openAccess ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.332FE643 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.actbio.2022.02.022 |
|---|