صورة
FIGURE 1 from FBXW7 -loss Sensitizes Cells to ATR Inhibition Through Induced Mitotic Catastrophe
| العنوان: | FIGURE 1 from FBXW7 -loss Sensitizes Cells to ATR Inhibition Through Induced Mitotic Catastrophe |
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| المؤلفون: | Siobhan O'Brien, Tajinder Ubhi, Lucie Wolf, Krishna Gandhi, Sichun Lin, Naz Chaudary, Neesha C. Dhani, Michael Milosevic, Grant W. Brown, Stephane Angers |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cancer, Cancer Biology, Molecular and Cellular Biology, Therapeutic Research and Development, Clinical Research and Trials, Oncogenes & Tumor Suppressors, Tumor suppressor genes, Cell Cycle, Control of cell cycle progression, Cell cycle checkpoints, Dna Damage And Repair, DNA repair, Drug Mechanisms, Cellular responses to anticancer drugs, Small Molecule Agents, Kinase inhibitors, Precision Medicine |
| الوصف: | Isogenic genome-wide screen identifies DNA repair genes essential following FBXW7 -loss. A, Schematic of genome-wide CRISPR screens in FBXW7 − / − and wild-type cells. B, Rank order plot summarizing the results of genome-wide screens highlighting the identification of various DNA damage response genes as selectively essential in FBXW7 − / − HPAF-II cells. BF = Bayes factor, a measure of gene fitness defects upon perturbation. C, Western blot analysis of pCHK1 (S345) identifies increased levels of replication stress in FBXW7 − / − compared with parental wild-type HPAF-II cells. Representative images of three independent replicate experiments. D, Quantification of pCHK1 (S345)/CHK1 in HPAF-II wild-type and FBXW7 − / − cells from C, n = 3, Students t test. E, Dot plots of two-dimensional cell-cycle flow cytometry assessing DNA content and EdU in wild-type and FBXW7 − / − HPAF-II cells. Representative images of three replicate experiments. F, Quantification of percent of cells EdU+ cells in D, n = 3, Students t test. G, Quantification of mean fluorescent intensity (MFI) of EdU+ cells from D, n = 3, Students t test. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/figure/FIGURE_1_from_i_FBXW7_i_-loss_Sensitizes_Cells_to_ATR_Inhibition_Through_Induced_Mitotic_Catastrophe/24886237Test |
| DOI: | 10.1158/2767-9764.24886237.v1 |
| الإتاحة: | https://doi.org/10.1158/2767-9764.24886237.v1Test https://figshare.com/articles/figure/FIGURE_1_from_i_FBXW7_i_-loss_Sensitizes_Cells_to_ATR_Inhibition_Through_Induced_Mitotic_Catastrophe/24886237Test |
| حقوق: | CC BY |
| رقم الانضمام: | edsbas.330F018A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1158/2767-9764.24886237.v1 |
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