التفاصيل البيبلوغرافية
| العنوان: |
SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA |
| المؤلفون: |
Jack, Amanda, Ferro, Luke S., Trnka, Michael J., Wehri, Eddie, Nadgir, Amrut, Nguyenla, Xammy, Fox, Douglas, Costa, Katelyn, Stanley, Sarah, Schaletzky, Julia, Yildiz, Ahmet |
| المساهمون: |
Cimarelli, Andrea, Office of the Director, National Institute of General Medical Sciences, Directorate for Biological Sciences, Dr. Miriam and Sheldon G. Adelson Medical Research Foundation, the UCSF Program for Biomedical Breakthrough Research, The Center for Rare and Neglected Diseases at UC Berkeley, Fast Grants |
| المصدر: |
PLOS Biology ; volume 19, issue 10, page e3001425 ; ISSN 1545-7885 |
| بيانات النشر: |
Public Library of Science (PLoS) |
| سنة النشر: |
2021 |
| المجموعة: |
PLOS Publications (via CrossRef) |
| الوصف: |
The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection causes Coronavirus Disease 2019 (COVID-19), a pandemic that seriously threatens global health. SARS-CoV-2 propagates by packaging its RNA genome into membrane enclosures in host cells. The packaging of the viral genome into the nascent virion is mediated by the nucleocapsid (N) protein, but the underlying mechanism remains unclear. Here, we show that the N protein forms biomolecular condensates with viral genomic RNA both in vitro and in mammalian cells. While the N protein forms spherical assemblies with homopolymeric RNA substrates that do not form base pairing interactions, it forms asymmetric condensates with viral RNA strands. Cross-linking mass spectrometry (CLMS) identified a region that drives interactions between N proteins in condensates, and deletion of this region disrupts phase separation. We also identified small molecules that alter the size and shape of N protein condensates and inhibit the proliferation of SARS-CoV-2 in infected cells. These results suggest that the N protein may utilize biomolecular condensation to package the SARS-CoV-2 RNA genome into a viral particle. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1371/journal.pbio.3001425 |
| الإتاحة: |
https://doi.org/10.1371/journal.pbio.3001425Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.327F88ED |
| قاعدة البيانات: |
BASE |
