دورية أكاديمية
Multiplexed plasmonic nano-labeling for bioimaging of cytological stained samples
| العنوان: | Multiplexed plasmonic nano-labeling for bioimaging of cytological stained samples |
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| المؤلفون: | Marcoux-Valiquette, Paule, Darviot, Cécile, Wang, Lu, Grosset, Andrée-Anne, Hasanzadeh Kafshgari, Morteza, Birela, Mirela, Patskovsky, Sergiy, Trudel, Dominique, Meunier, Michel |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | École Polytechnique de Montréal: PolyPublie |
| مصطلحات موضوعية: | 1900 Génie biomédical, 1901 Technologie biomédicale, 3100 Physique, 3101 Études atomiques et moléculaires, 3110 Optique (voir aussi Dispositifs photoniques, 2505) |
| الوصف: | Reliable cytopathological diagnosis requires new methods and approaches for the rapid and accurate determination of all cell types. This is especially important when the number of cells is limited, such as in the cytological samples of fine-needle biopsy. Immunoplasmonic-multiplexedlabeling may be one of the emerging solutions to such problems. However, to be accepted and used by the practicing pathologists, new methods must be compatible and complementary with existing cytopathology approaches where counterstaining is central to the correct interpretation of immunolabeling. In addition, the optical detection and imaging setup for immunoplasmonicmultiplexed-labeling must be implemented on the same cytopathological microscope, not interfere with standard H&E imaging, and operate as a second easy-to-use imaging method. In this article, we present multiplex imaging of four types of nanoplasmonic markers on two types of H&E-stained cytological specimens (formalin-fixed paraffin embedded and non-embedded adherent cancer cells) using a specially designed adapter for SI dark-field microscopy. The obtained results confirm the effectiveness of the proposed optical method for quantitative and multiplex identification of various plasmonic NPs, and the possibility of using immunoplasmonic-multiplexed-labeling for cytopathological diagnostics. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://publications.polymtl.ca/9370/1/2021_Marcoux-Valiquette_Multiplexed_Plasmonic_Nano-Labeling_Bioimaging_Cytological.pdfTest; Marcoux-Valiquette, P., Darviot, C., Wang, L., Grosset, A.-A., Hasanzadeh Kafshgari, M., Birela, M., Patskovsky, S., Trudel, D., & Meunier, M. (2021). Multiplexed plasmonic nano-labeling for bioimaging of cytological stained samples. Cancers, 13(14), 3509 (10 pages). |
| DOI: | 10.3390/cancers13143509 |
| الإتاحة: | https://doi.org/10.3390/cancers13143509Test https://publications.polymtl.ca/9370Test/ https://publications.polymtl.ca/9370/1/2021_Marcoux-Valiquette_Multiplexed_Plasmonic_Nano-Labeling_Bioimaging_Cytological.pdfTest |
| حقوق: | cc_by |
| رقم الانضمام: | edsbas.320FC1CE |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cancers13143509 |
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