دورية أكاديمية
Characterization of ectopic lymphoid structures in different types of acute renal allograft rejection
| العنوان: | Characterization of ectopic lymphoid structures in different types of acute renal allograft rejection |
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| المؤلفون: | de Leur, K, Clahsen-van Groningen, M C, van den Bosch, T P P, de Graav, G N, Hesselink, D A, Samsom, J N, Baan, C C, Boer, K |
| المساهمون: | Erasmus MC, University Medical Center |
| المصدر: | Clinical and Experimental Immunology ; volume 192, issue 2, page 224-232 ; ISSN 0009-9104 1365-2249 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2018 |
| الوصف: | Summary We hypothesize that T cells such as interleukin (IL)-21+B cell lymphoma 6 (BCL6)+ T follicular helper cells can regulate B cell-mediated immunity within the allograft during acute T cell-mediated rejection; this process may feed chronic allograft rejection in the long term. To investigate this mechanism, we determined the presence and activation status of organized T and B cells in so-called ectopic lymphoid structures (ELSs) in different types of acute renal allograft rejection. Biopsies showing the following primary diagnosis were included: acute/active antibody-mediated rejection, C4d+ (a/aABMR), acute T cell-mediated rejection grade I (aTCMRI) and acute T cell-mediated rejection grade II (aTCMRII). Paraffin sections were stained for T cells (CD3 and CD4), B cells (CD20), follicular dendritic cells (FDCs, CD23), activated B cells (CD79A), immunoglobulin (Ig)D, cell proliferation (Ki67) and double immunofluorescent stainings for IL-21 and BCL6 were performed. Infiltrates of T cells were detected in all biopsies. In aTCMRI, B cells formed aggregates surrounded by T cells. In these aggregates, FDCs, IgD and Ki67 were detected, suggesting the presence of ELSs. In contrast, a/aABMR and aTCMRII showed diffuse infiltrates of T and B cells but no FDCs and IgD. IL-21 was present in all biopsies. However, co-localization with BCL6 was observed mainly in aTCMRI biopsies. In conclusion, ELSs with an activated phenotype are found predominantly in aTCMRI where T cells co-localize with B cells. These findings suggest a direct pathway of B cell alloactivation at the graft site during T cell mediated rejection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/cei.13099 |
| الإتاحة: | https://doi.org/10.1111/cei.13099Test https://academic.oup.com/cei/article-pdf/192/2/224/41644786/cei13099.pdfTest |
| حقوق: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_modelTest |
| رقم الانضمام: | edsbas.32014646 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/cei.13099 |
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