دورية أكاديمية
RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulators.
| العنوان: | RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulators. |
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| المؤلفون: | Li, Bin, Liu, Shurong, Zheng, Wujian, Liu, Anrui, Yu, Peng, Wu, Di, Zhou, Jie, Zhang, Ping, Liu, Chang, Lin, Qiao, Ye, Jiayi, He, Simeng, Huang, Qiaojuan, Zhou, Hui, Chen, Jianjun, Qu, Lianghu, Yang, Jianhua |
| المصدر: | Nat Biotechnol ; ISSN:1546-1696 ; Volume:42 ; Issue:1 |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| الوصف: | A kink-turn (K-turn) is a three-dimensional RNA structure that exists in all three primary phylogenetic domains. In this study, we developed the RIP-PEN-seq method to identify the full-length sequences of RNAs bound by the K-turn binding protein 15.5K and discovered a previously uncharacterized class of RNAs with backward K-turn motifs (bktRNAs) in humans and mice. All bktRNAs share two consensus sequence motifs at their fixed terminal position and have complex folding properties, expression and evolution patterns. We found that a highly conserved bktRNA1 guides the methyltransferase fibrillarin to install RNA methylation of U12 small nuclear RNA in humans. Depletion of bktRNA1 causes global splicing dysregulation of U12-type introns by impairing the recruitment of ZCRB1 to the minor spliceosome. Most bktRNAs regulate the splicing of local introns by interacting with the 15.5K protein. Taken together, our findings characterize a class of small RNAs and uncover another layer of gene expression regulation that involves crosstalk among bktRNAs, RNA splicing and RNA methylation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1038/s41587-023-01749-0Test; https://pubmed.ncbi.nlm.nih.gov/37037902Test |
| DOI: | 10.1038/s41587-023-01749-0 |
| الإتاحة: | https://doi.org/10.1038/s41587-023-01749-0Test https://pubmed.ncbi.nlm.nih.gov/37037902Test |
| حقوق: | © 2023. The Author(s), under exclusive licence to Springer Nature America, Inc. |
| رقم الانضمام: | edsbas.31914CA3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41587-023-01749-0 |
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