صورة
Image_5_Prediabetes Induced by a Single Autoimmune B Cell Clone.TIF
| العنوان: | Image_5_Prediabetes Induced by a Single Autoimmune B Cell Clone.TIF |
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| المؤلفون: | Nathaniel Phillips, Eugene Ke, Amy Nham, Maximilian Seidl, Brent Freeman, Justin R. Abadejos, Changchun Xiao, David Nemazee, Manching Ku, Oktay Kirak |
| سنة النشر: | 2020 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, diabetes, B cell, animal model, pancreas, cloning, somatic cell nuclear transfer (SCNT), nucleic acid sensing |
| الوصف: | While B cells play a significant role in the onset of type-1 diabetes (T1D), little is know about their role in those early stages. Thus, to gain new insights into the role of B cells in T1D, we converted a physiological early pancreas-infiltrating B cell into a novel BCR mouse model using Somatic Cell Nuclear Transfer (SCNT). Strikingly, SCNT-derived B1411 model displayed neither developmental block nor anergy. Instead, B1411 underwent spontaneous germinal center reactions. Without T cell help, B1411-Rag1 −/− was capable of forming peri-/intra-pancreatic lymph nodes, and undergoing class-switching. RNA-Seq analysis identified 93 differentially expressed genes in B1411 compared to WT B cells, including Irf7, Usp18, and Mda5 that had been linked to a potential viral etiology of T1D. We also found various members of the oligoadenylate synthase (OAS) family to be enriched in B1411, such as Oas1, which had recently also been linked to T1D. Strikingly, when challenged with glucose B1411-Rag1 −/− mice displayed impaired glucose tolerance. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/figure/Image_5_Prediabetes_Induced_by_a_Single_Autoimmune_B_Cell_Clone_TIF/12503849Test |
| DOI: | 10.3389/fimmu.2020.01073.s005 |
| الإتاحة: | https://doi.org/10.3389/fimmu.2020.01073.s005Test https://figshare.com/articles/figure/Image_5_Prediabetes_Induced_by_a_Single_Autoimmune_B_Cell_Clone_TIF/12503849Test |
| حقوق: | CC BY 4.0 |
| رقم الانضمام: | edsbas.30FE1EF8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2020.01073.s005 |
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