التفاصيل البيبلوغرافية
| العنوان: |
A guide to studying human dermal adipocytes in situ |
| المؤلفون: |
Nicu, Carina, Pople, Jennifer, Bonsell, Laura, Bhogal, Ranjit, Ansell, David M., Paus, Ralf |
| المساهمون: |
National Institute on Handicapped Research, Biotechnology and Biological Sciences Research Council |
| المصدر: |
Experimental Dermatology ; volume 27, issue 6, page 589-602 ; ISSN 0906-6705 1600-0625 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2018 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Dermal white adipose tissue ( DWAT ) is a main component of human skin, composed of individual lipid‐laden mesenchymal cells known as dermal adipocytes ( DA s). Besides their well‐known role in lipid storage and release, DA s also promote skin immunity, wound healing and hair follicle cycling and are important players in cutaneous neuroendocrinology. The ever‐growing insights into DWAT functions, albeit mostly in mice, have invited speculation that it may be involved in multiple skin diseases ranging from fibrosis to alopecia and psoriasis, thus designating human DWAT a clinically relevant, but as yet insufficiently investigated skin compartment. Therefore, this practical, user‐friendly guide aims to introduce the techniques available to study human DWAT in situ and ex vivo, including immunohistochemistry, immunofluorescence microscopy and analysis via quantitative immunohistomorphometry. Here, we provide information on a collection of stains comprising pre‐adipocyte (Pref1) and mature adipocyte markers (Perilipin1, Caveolin1), as well as various lipid (OilRedO, BODIPY ) and histochemical stains (H&E, trichrome) available for use on human DWAT . We offer the reader guidelines on fixing, processing and staining human DA s and highlight caveats and solutions to common problems that one may encounter when studying this fascinating skin compartment. We also suggest standard methods for conducting quantitative immunohistomorphometry on human DWAT and its individual adipocytes to quantify cell size, number, lipid content and fluorescence intensity of adipose‐specific markers. Finally, we briefly introduce in situ hybridization, transmission electron microscopy and essentials of magnetic resonance imaging imaging as additional tools for instructively interrogating this largest, but still least‐known compartment of human skin. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/exd.13549 |
| الإتاحة: |
https://doi.org/10.1111/exd.13549Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.2FD886ED |
| قاعدة البيانات: |
BASE |
