دورية أكاديمية
Exploring the Physical Properties of Lipid Membranes with Polyhydroxy Oxanorbornane Head Group Using NBD-Conjugated and DPH Fluorescent Probes
| العنوان: | Exploring the Physical Properties of Lipid Membranes with Polyhydroxy Oxanorbornane Head Group Using NBD-Conjugated and DPH Fluorescent Probes |
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| المؤلفون: | Anand Kumar Sahu, U. Chandrasekhar Reddy, Muraleedharan Kannoth Manheri, Ashok Kumar Mishra |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Medicine, Biotechnology, Evolutionary Biology, Ecology, Environmental Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, two hydroxy groups, hydroxy groups present, hydrogen bonding interactions, different solvent environments, lipid head groups, study two variants, present study focuses, effective synthetic alternative, archaeal lipid membranes, 74 ± 1, 68 ± 1, size ), fluidity, phase transition temperature, based lipids differing, lipid membranes based, based lipid membranes, 3oh lipid membranes, resolved fluorescence properties, dph fluorescent probes |
| الوصف: | The present study focuses on exploring the physical properties of lipid membranes based on the polyhydroxy oxanorbornane (PH-ONB) headgroup, designed as synthetic analogues of naturally occurring archaeal lipid membranes. Specifically, we study two variants of PH-ONB headgroup-based lipids differing in the number of hydroxy groups present in the headgroup, with one having two hydroxy groups (ONB-2OH) and the other having three (ONB-3OH). These lipids form stable bilayer membranes. The study begins with a comprehensive analysis of the fluorescence characteristics of nitrobenzoxadiazole (NBD)-tagged ONB-based lipids in different solvent environments and within a model lipid membrane 1,2-dimyristoyl- sn -glycero-3-phosphocholine (DMPC). Subsequently, the physical properties of the ONB-based membranes were examined by using an NBD-tagged ONB-based probe and a commonly used extrinsic 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescent probe. The steady-state and time-resolved fluorescence properties of the NBD-tagged ONB-based probe and DPH were used to compare the physical properties of the ONB-based membranes, including polarity, fluidity, phase transition, order, hydration, location, heterogeneity, and rotational diffusion. The solid gel to liquid crystalline phase transition temperatures of ONB-2OH and ONB-3OH lipid membranes are found to be (68 ± 1) °C and (74 ± 1) °C, respectively. The variation in organization (size), fluidity, and phase transition temperature of ONB-based lipid membranes is explained by the extent of hydrogen bonding interactions between lipid head groups. ONB-based membranes exhibit characteristics similar to those of phospholipid membranes and possess a notably high phase transition temperature. These properties make them a promising and cost-effective synthetic alternative to archaeal lipid membranes with a wide range of potential applications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Exploring_the_Physical_Properties_of_Lipid_Membranes_with_Polyhydroxy_Oxanorbornane_Head_Group_Using_NBD-Conjugated_and_DPH_Fluorescent_Probes/25328680Test |
| DOI: | 10.1021/acs.langmuir.3c02941.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.langmuir.3c02941.s001Test https://figshare.com/articles/journal_contribution/Exploring_the_Physical_Properties_of_Lipid_Membranes_with_Polyhydroxy_Oxanorbornane_Head_Group_Using_NBD-Conjugated_and_DPH_Fluorescent_Probes/25328680Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.2F4971DB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.langmuir.3c02941.s001 |
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