دورية أكاديمية
Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling
| العنوان: | Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling |
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| المؤلفون: | Lifang Zhang, Han Xu, Xuhui Zhang, Xinxin Chen, Yanbing Lv, Ruixue Zhang, Lei Wang, Ruili Wu, Huaibin Shen, Lin Song Li |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Cell Biology, Biotechnology, Ecology, Inorganic Chemistry, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, photoluminescence quantum yield, phase transfer process, linked immunosorbent assay, lattice stress relaxation, biotin – streptavidin, biocompatible environments remains, bio – sa, amphiphilic polymer encapsulation, stable pl intensity, nanostructure tailoring revealed, fluorescent immunoassay probes, thick shell growth, znses alloy layer, high optical stability, nanostructure tailoring, alloy shell, thick shell, quantitative immunoassay, based probes, developed thick, shell qds |
| الوصف: | Nontoxic, highly sensitive InP quantum dot (QD) fluorescent immunoassay probes are promising biomedical detection modalities due to their unique properties. However, InP-based QDs are prone to surface oxidation, and the stability of InP QD-based probes in biocompatible environments remains a crucial challenge. Although the thick shell can provide some protection during the phase transfer process of hydrophobic QDs, the photoluminescence quantum yield (PLQY) is generally decreased because of the contradiction between lattice stress relaxation and thick shell growth. Herein, we developed thick-shell InP-based core/shell QDs by inserting a ZnSeS alloy layer. The ternary ZnSeS intermediate shell could effectively facilitate lattice stress relaxation and passivate the defect states. The synthesized InP/ZnSe/ZnSeS/ZnS core/alloy shell/shell QDs (CAS-InP QDs) with nanostructure tailoring revealed a larger size, high PLQY (90%), and high optical stability. After amphiphilic polymer encapsulation, the aqueous CAS-InP QDs presented almost constant fluorescence attenuation and stable PL intensity under different temperatures, UV radiation, and pH solutions. The CAS-InP QDs were excellent labels of the fluorescence-linked immunosorbent assay (FLISA) for detecting C-reactive protein (CRP). The biotin–streptavidin (Bio–SA) system was first introduced in the FLISA to further improve the sensitivity, and the CAS-InP QDs-based SA–Bio sandwich FLISA realized the detection of CRP with an impressive limit of detection (LOD) of 0.83 ng/mL. It is believed that the stable and sensitive InP QD fluorescent probes will drive the rapid development of future eco-friendly, cost-effective, and sensitive in vitro diagnostic kits. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Highly_Sensitive_Stable_InP_Quantum_Dot_Fluorescent_Probes_for_Quantitative_Immunoassay_Through_Nanostructure_Tailoring_and_Biotin_Streptavidin_Coupling/25282682Test |
| DOI: | 10.1021/acs.inorgchem.3c04153.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.inorgchem.3c04153.s001Test https://figshare.com/articles/journal_contribution/Highly_Sensitive_Stable_InP_Quantum_Dot_Fluorescent_Probes_for_Quantitative_Immunoassay_Through_Nanostructure_Tailoring_and_Biotin_Streptavidin_Coupling/25282682Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.2F163B97 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.inorgchem.3c04153.s001 |
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